Soybean Research Institute, Shenyang Agricultural University, Shenyang, 110866, China.
Yantai High-tech International Science and Technology Cooperation, Yantai, Shandong, China.
Ecotoxicol Environ Saf. 2019 Jul 15;175:90-101. doi: 10.1016/j.ecoenv.2019.03.042. Epub 2019 Mar 16.
Recent climatic changes and low water availability due to unpredictable precipitation have reduced the productivity of soybean (Glycine max [L.] Merr.) cultivars. Limited information is available on how drought affects the accumulation and translocation of cadmium (Cd) by affecting soybean root. In this study, we investigated the effect of polyethylene glycol (PEG; 5% and 10%)-induced drought and Cd (0.2 and 0.5 mg L) stresses on soybean root morphology, Cd uptake and gene expression; plants not exposed to these stress (0% PEG and 0 mg L Cd) served as a control. The results showed that drought affected roots morphology and Cd uptake. The reduction in root length, root area and root diameter and increase in catalase activity was less prominent in drought tolerant cultivars (Shennong20 and Liaodou32) than in drought sensitive cultivars (Liaodou3 and Liaodou10). Genes involved in abscisic acid (ABA) degradation, gibberellin and salicylic acid biosynthesis, hydrogen peroxide (HO) production and Cd transport were up-regulated, while those involved in zeatinriboside (ZR), indole 3-acetic acid (IAA) and methyl jasmonate (MeJA) biosynthesis were down-regulated under Cd and drought stress. Biosynthesis genes of gibberellin (Glyma03G019800.1), IAA (Glyma02G037600), ZR (XM_003550461.3) and MeJA (Glyma11G007600) were expressed to higher levels in drought tolerant cultivars than in drought sensitive cultivars. These genes represent potential candidates for the development of drought and Cd tolerant soybean cultivars.
最近的气候变化和不可预测降水导致的水资源短缺降低了大豆（Glycine max [L.] Merr.）品种的生产力。关于干旱如何通过影响大豆根系来影响镉（Cd）的积累和转运，相关信息有限。在这项研究中，我们研究了聚乙二醇（PEG；5%和 10%）诱导的干旱和 Cd（0.2 和 0.5 mg/L）胁迫对大豆根系形态、Cd 吸收和基因表达的影响；未暴露于这些胁迫（0%PEG 和 0 mg/L Cd）的植物作为对照。结果表明，干旱影响根系形态和 Cd 吸收。与干旱敏感品种（Liaodou3 和 Liaodou10）相比，耐旱品种（Shennong20 和 Liaodou32）的根长、根面积、根直径减少和过氧化氢酶活性增加不明显。参与脱落酸（ABA）降解、赤霉素和水杨酸生物合成、过氧化氢（HO）产生和 Cd 转运的基因上调，而参与玉米素核苷（ZR）、吲哚 3-乙酸（IAA）和茉莉酸甲酯（MeJA）生物合成的基因下调在 Cd 和干旱胁迫下。赤霉素（Glyma03G019800.1）、IAA（Glyma02G037600）、ZR（XM_003550461.3）和 MeJA（Glyma11G007600）的生物合成基因在耐旱品种中的表达水平高于干旱敏感品种。这些基因可能是培育耐旱和耐 Cd 大豆品种的候选基因。
