Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, PR China.
Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, PR China.
Chemosphere. 2019 Jun;225:424-433. doi: 10.1016/j.chemosphere.2019.03.070. Epub 2019 Mar 12.
Microcystis aeruginosa (M. aeruginosa), as the dominant algae in eutrophic water bodies, has caused a serious harm to the local eco-environment. A biological tool, employing allelopathic inhibitory of eucalyptus to control M. aeruginosa, has been receiving tremendous attention. This work presents the results of the allelopathic inhibitory effects of eucalyptus (Eucalyptus grandis × E.urophylla 'GLGU9') extracts of roots (ERE), stems (ESE), and leaves (ELE) on culture solutions of M. aeruginosa and its eco-physiological mechanism. The inhibitory effects of the extracts on the growth of M. aeruginosa varied greatly with ELE exhibiting the highest level of potency. Modes of action by which ELE inhibited M. aeruginosa growth were established. They involved reduction in photosynthesis, disruption of the cell membrane integrity, and inhibition of esterase activities of the cyanobacterial cells. However, ELE did not exhibit any gradients of toxicity towards zebrafish nor Washington grass plant. Species abundance and diversity in the systems remained likewise unaffected by ELE. The synergistic interaction between ELE and single-component allelochemicals (e.g., gallic acid and berberine) was ascribed to the increase in efficacy of allelochemicals in the various systems. The results of this study provide an underlying, novel, and attractive approach for controlling the growth of M. aeruginosa in aquatic environments.
铜绿微囊藻（M. aeruginosa）作为富营养化水体中的优势藻类，对当地生态环境造成了严重危害。桉树的化感抑制作用作为一种生物工具，已被广泛用于控制铜绿微囊藻。本研究旨在探讨桉树（巨桉×尾巨桉‘GLGU9’）根（ERE）、茎（ESE）和叶（ELE）提取物对铜绿微囊藻培养液的化感抑制作用及其生态生理机制。研究结果表明，提取物对铜绿微囊藻生长的抑制作用差异显著，其中 ELE 的抑制效果最强。ELE 抑制铜绿微囊藻生长的作用机制包括降低光合作用、破坏细胞膜完整性以及抑制蓝藻细胞的酯酶活性。然而，ELE 对斑马鱼和华盛顿草植物没有表现出任何毒性梯度，系统中的物种丰度和多样性也没有受到影响。ELE 与单一化感化合物（如没食子酸和小檗碱）之间的协同相互作用归因于各种系统中化感化合物功效的提高。本研究为控制水生环境中铜绿微囊藻的生长提供了一种新颖且有吸引力的方法。
