Key Laboratory of Resources Conversion and Pollution Control of the State Ethnic Affairs Commission, College of Resources and Environmental Science, South-Central Minzu University, Wuhan, 430074, People's Republic of China.
Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.
Sci Rep. 2022 May 16;12(1):8086. doi: 10.1038/s41598-022-12124-5.
Nowadays, the increasing Dolichospermum (Anabaena) blooms pose a major threat to the aquatic environment and public health worldwide. The use of naturally derived chemicals from plants to control cyanobacteria blooms has recently received a tremendous amount of attention. This study investigates the possibility of transforming watermelon peel (WMP) into a biological resource to allelopathically inhibit Dolichospermum flos-aquae blooms. The results demonstrated that the growth of D. flos-aquae was efficiently restricted by the aqueous extract of watermelon peel (WMPAE) in a concentration-dependent manner. Cell viability decreased quickly, intracellular structural damage occurred, chlorophyll a in algal cells degraded, and photosynthesis was clearly inhibited. At the same time, the levels of reactive oxygen species in viable cells increased significantly, as did malondialdehyde levels, indicating that WMPAE elucidated strong oxidative stress and corresponding damage to D. flos-aquae. Capsular polysaccharide (CPS) levels increased in all treatment groups, which represents an adaptive response indicative of the development of resistance to WMPAE stress and oxidative damage. Despite this, WMPAE had clear inhibitory effects on D. flos-aquae. These findings provide fundamental information on an allelopathic system that could be a novel and attractive approach for suppressing D. flos-aquae blooms in small aquatic environments, especially aquaculture ponds.
如今，不断增加的水华鱼腥藻（Anabaena）对全球的水生环境和公共健康构成了重大威胁。利用植物中天然衍生的化学物质来控制蓝藻水华已引起了广泛关注。本研究探讨了将西瓜皮（WMP）转化为生物资源，以化感抑制水华鱼腥藻水华的可能性。结果表明，西瓜皮水提物（WMPAE）以浓度依赖的方式有效地抑制了水华鱼腥藻的生长。细胞活力迅速下降，细胞内结构受损，藻类细胞中的叶绿素 a 降解，光合作用明显受到抑制。同时，活细胞中的活性氧水平显著升高，丙二醛水平也升高，表明 WMPAE 引发了强烈的氧化应激和相应的水华鱼腥藻损伤。所有处理组的囊泡多糖（CPS）水平都升高，这表明对 WMPAE 胁迫和氧化损伤产生了适应性反应，表明水华鱼腥藻产生了抗性。尽管如此，WMPAE 对水华鱼腥藻仍具有明显的抑制作用。这些发现为化感系统提供了基本信息，这可能是一种抑制小型水生环境，特别是水产养殖池塘中水华鱼腥藻水华的新颖而有吸引力的方法。
