一种用于痕量葡萄糖定量分析的简便 SERS 策略，该策略结合了葡萄糖氧化酶和纳米银对四甲基联苯胺的催化氧化作用。
A facile SERS strategy for quantitative analysis of trace glucose coupling glucose oxidase and nanosilver catalytic oxidation of tetramethylbenzidine.
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China; College of Chemistry and Biology Engineering, Hechi University, Yizhou 546300, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jun 5;216:146-153. doi: 10.1016/j.saa.2019.03.026. Epub 2019 Mar 12.
Highly stable, SERS active and catalytic nanosilver sol (AgNP) was synthesized under the exposure of light wave, using AgNO as precursor and sodium citrate as reducer. Under the conditions of pH 7.0 NaHPO-NaHPO buffer solution (PBS), the glucose can be catalyzed by glucose oxidase to produce HO specifically. Based on the nanocatalyst and SERS substrate of AgNP, HO can oxidize the 3,3',5,5'-tetramethylbenzidine (TMB) quickly to form a blue oxidation product (TMB) that induced the AgNPs aggregation, which exhibited a strong SERS signal at 1606 cm. As the concentration of glucose increases, the TMB molecular probes and AgNPs aggregation increase, and the intensity of SERS peak at 1606 cm increase linearly. Thus, a new SERS strategy for quantitative analysis of 0.33-6.67 μmol/L glucose was developed, with a detection limit of 0.035 μmol/L, coupled the catalysis of nanosilver with glucose oxidase, and label-free molecular probe of TMB.
利用 AgNO3 作为前驱体，以柠檬酸钠作为还原剂，在光波的照射下合成了具有高稳定性、表面增强拉曼散射（SERS）活性和催化作用的纳米银溶胶（AgNP）。在 pH 值为 7.0 的 NaHPO4-NaHPO4 缓冲溶液（PBS）条件下，葡萄糖可以被葡萄糖氧化酶特异性地催化生成 HO。基于纳米催化剂和 AgNP 的 SERS 基底，HO 可以迅速氧化 3,3',5,5'-四甲基联苯胺（TMB），形成蓝色氧化产物（TMB），导致 AgNPs 聚集，在 1606 cm-1 处表现出强的 SERS 信号。随着葡萄糖浓度的增加，TMB 分子探针和 AgNPs 聚集增加，在 1606 cm-1 处的 SERS 峰强度呈线性增加。因此，开发了一种新的用于定量分析 0.33-6.67 μmol/L 葡萄糖的 SERS 策略，检测限低至 0.035 μmol/L，该策略结合了纳米银的催化作用与葡萄糖氧化酶和 TMB 的无标记分子探针。
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