基于 FeMOF 催化氧化四甲基联苯胺的痕量草甘膦适体三模态生物传感器。

Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine.

机构信息

School of Public Health, Guilin Medical University, Guilin 541199, China.

Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541006, China.

出版信息

Biosensors (Basel). 2022 Oct 25;12(11):920. doi: 10.3390/bios12110920.

The stable and highly catalytic Fe metal-organic framework (FeMOF) nanosol was prepared and characterized by electron microscopy, and energy and molecular spectral analysis. It was found that FeMOF strongly catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce TMBox, which had a fluorescence (FL) peak at 410 nm. When silver nanoparticles were added, it exhibited strong resonance Rayleigh scattering (RRS) activity and surface-enhanced Raman scattering (SERS) effect. This new FeMOF nanocatalytic trimode indicator reaction was combined with the glyphosate aptamer reaction to establish a new SERS/RRS/FL trimode biosensor for glyphosate. The sensor can be used for the analysis of environmental wastewater, and a new method for detecting glyphosate content in wastewater is proposed. The linear range of the sensor is 0.1-14 nmol/L, the detection limit is 0.05 nmol/L, the recovery is 92.1-97.5%, and the relative standard deviation is 3.6-8.7%.

制备并通过电子显微镜、能量和分子光谱分析对稳定且高催化活性的铁金属有机骨架（FeMOF）纳米溶胶进行了表征。结果表明，FeMOF 可强烈催化 HO 对 3,3',5,5'-四甲基联苯胺（TMB）的氧化生成具有 410nm 荧光（FL）峰的 TMBox。加入银纳米粒子后，其表现出强共振瑞利散射（RRS）活性和表面增强拉曼散射（SERS）效应。将这种新的 FeMOF 纳米催化三模指示剂反应与草甘膦适体反应相结合，建立了一种用于草甘膦的新型 SERS/RRS/FL 三模生物传感器。该传感器可用于环境废水中的分析，并提出了一种用于检测废水中草甘膦含量的新方法。该传感器的线性范围为 0.1-14nmol/L，检测限为 0.05nmol/L，回收率为 92.1-97.5%，相对标准偏差为 3.6-8.7%。