血清 microRNAs 与慢性牙周炎：病例对照研究。
Serum microRNAs and chronic periodontitis: A case-control study.
机构信息
Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
Department of Community Oral Health, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, 501-0296, Japan.
出版信息
Arch Oral Biol. 2019 May;101:57-63. doi: 10.1016/j.archoralbio.2019.03.009. Epub 2019 Mar 13.
OBJECTIVE
An association is present between periodontitis and rates of expression of certain microRNAs (miRNAs) in periodontal tissue. However, the association between periodontitis and miRNA levels in human serum is unknown. We performed a case-control study in patients with chronic periodontitis to investigate serum miRNA levels.
DESIGN
We enrolled 30 healthy patients without periodontitis and 30 patients with chronic periodontitis. Participants underwent clinical examination, case selection, and a blood draw from the antecubital vein. Serum miRNA profiles were compared in samples from participants with and without chronic periodontitis using microarray and real-time PCR.
RESULTS
Microarray demonstrated seven miRNAs that were expressed <1/1.5 or >1.5 in the control group compared to the periodontitis group (p < 0.05). Real-time PCR showed that hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p were higher in the periodontitis group than the control group (p < 0.05).
CONCLUSION
Hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p are candidate serum biomarkers for chronic periodontitis.
目的
牙周炎与牙周组织中某些 microRNAs（miRNAs）的表达率之间存在关联。然而，牙周炎与人类血清中 miRNA 水平之间的关联尚不清楚。我们对患有慢性牙周炎的患者进行了病例对照研究，以调查血清 miRNA 水平。
设计
我们招募了 30 名无牙周炎的健康患者和 30 名患有慢性牙周炎的患者。参与者接受了临床检查、病例选择和肘前静脉采血。使用微阵列和实时 PCR 比较了慢性牙周炎患者和对照组血清样本中的血清 miRNA 谱。
结果
微阵列显示，与牙周炎组相比，对照组中有 7 种 miRNA 的表达<1/1.5 或>1.5（p<0.05）。实时 PCR 显示，hsa-miR-664a-3p、hsa-miR-501-5p 和 hsa-miR-21-3p 在牙周炎组中的表达高于对照组（p<0.05）。
结论
hsa-miR-664a-3p、hsa-miR-501-5p 和 hsa-miR-21-3p 是慢性牙周炎的候选血清生物标志物。
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