Division of Restorative Dentistry, Department of Ecological Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan.
BMC Oral Health. 2023 Mar 30;23(1):187. doi: 10.1186/s12903-023-02874-7.
There is increasing evidence that diagnostic salivary tests measuring inflammatory biomarkers are being developed to assess inflammatory status for early detection, prevention, and progression of periodontal disease. Therefore, the aim of the present study was to investigate and identify the salivary biomarker that can predict the inflammatory status of periodontal disease.
A total of 36 patients (28 women and 8 men) with an average age of 57 years were investigated. Unstimulated saliva was collected from the recruited subjects and analyzed using SillHa, a saliva-testing device that measures bacteria count, saliva buffer capacity, acidity, leukocyte esterase, protein, and ammonia. Periodontal parameters were then obtained by clinical examination and initial periodontal therapy was performed. Data obtained with SillHa were compared with clinical periodontal parameters at baseline, re-examination (three months from baseline), and final examination (six months from re-examination).
Leukocyte esterase activity in saliva measured by SillHa; BOP and PCR measured by clinical examination showed a significant difference between baseline and final examination and between re-examination and final examination. Patients in the lower median group (group 1) had a significant difference in leukocyte esterase activity between baseline and final examination and re-examination and final examination. In addition, patients in Group 1 had significantly lower BOP between baseline and final examination. While patients in the higher median group (group 2) showed a modest decrease in leukocyte esterase activity, which was significant only between baseline and final examination, no significant changes were observed concerning BOP. Furthermore, the associated systemic disease was observed in 30% and 81.2% of group 1 and 2 patients, respectively.
The results suggest that leukocyte esterase activity in saliva measured by SillHa could serve as a reliable diagnostic marker for monitoring inflammatory status in periodontal disease.
越来越多的证据表明，正在开发测量炎症生物标志物的诊断性唾液测试，以评估牙周病的炎症状态，从而进行早期检测、预防和进展。因此，本研究旨在调查和确定可预测牙周病炎症状态的唾液生物标志物。
共调查了 36 名（28 名女性和 8 名男性）平均年龄为 57 岁的患者。从招募的受试者中采集未刺激的唾液，并使用 SillHa 进行分析，该设备可测量细菌计数、唾液缓冲能力、酸度、白细胞酯酶、蛋白质和氨。然后通过临床检查获得牙周参数，并进行初始牙周治疗。使用 SillHa 获得的数据与基线、复查（从基线起三个月）和最终检查（从复查起六个月）的临床牙周参数进行比较。
SillHa 测量的唾液白细胞酯酶活性；临床检查测量的 BOP 和 PCR 在基线和最终检查以及复查和最终检查之间均显示出显著差异。白细胞酯酶活性在较低中位数组（第 1 组）中，在基线和最终检查以及复查和最终检查之间存在显著差异。此外，第 1 组患者的 BOP 在基线和最终检查之间显著降低。而在较高中位数组（第 2 组）中，白细胞酯酶活性略有下降，但仅在基线和最终检查之间具有统计学意义，BOP 无明显变化。此外，第 1 组和第 2 组患者的相关系统性疾病分别为 30%和 81.2%。
研究结果表明，SillHa 测量的唾液白细胞酯酶活性可以作为监测牙周病炎症状态的可靠诊断标志物。
