Department of Pharmacy, Shri Venkateshwara University, NH-24, Rajabpur, Gajraula, Amroha 244236, Uttar Pradesh, India.
Quality Assurance Laboratory, Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110025, India.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1105-1114. doi: 10.1016/j.msec.2019.02.061. Epub 2019 Feb 15.
The present research work describes a novel method for green synthesis of silver nanoparticles using purple heart plant leaves extract, which is recognized as frequently found in households as an ornamental plant. The aqueous methanolic extract of purple heart plant leaves was prepared and employed in the synthesis of stable silver nanoparticles via biological reduction method. The purple heart plant leaves extract-mediated synthesized silver nanoparticles were systematically optimized using Box-Behnken design considering the effect of various independent variables (factors) like concentration of AgNO, temperature and volume of purple heart plant leaves extract solution on the responses like particle size and polydispersity index of synthesized silver nanoparticles were optimized. Mathematical modelling was performed using quadratic polynomial model and response surface analysis was done to understand the factor-response relationship. The synthesized silver nanoparticles at optimum condition were found to be of spherical in shape under TEM with particle size of 98 nm and polydispersity index of 0.15. Optimized silver nanoparticles were further characterized through UV-VIS spectrophotometry, FTIR spectroscopy and TEM imaging studies. Also, the silver nanoparticles were evaluated for antibacterial activity on E. coli and S. aureus. In a nutshell, the studies construed successful synthesis of silver nanoparticles along with thorough understanding of the associated factors influencing their quality characteristics and significantly improved antibacterial activity as beneficial effect.
本研究工作描述了一种使用紫心植物叶片提取物绿色合成银纳米粒子的新方法，紫心植物叶片提取物已被广泛用作家庭观赏植物。采用生物还原法，以紫心植物叶片的水-甲醇提取物为原料，合成了稳定的银纳米粒子。通过 Box-Behnken 设计，系统优化了紫心植物叶片提取物介导的银纳米粒子合成，考虑了不同独立变量（因素）如 AgNO 的浓度、温度和紫心植物叶片提取物溶液的体积对合成银纳米粒子的粒径和多分散指数等响应的影响。采用二次多项式模型进行数学建模，并进行响应面分析以了解因素-响应关系。在 TEM 下，在最佳条件下合成的银纳米粒子呈球形，粒径为 98nm，多分散指数为 0.15。通过紫外可见分光光度法、傅里叶变换红外光谱法和 TEM 成像研究对优化后的银纳米粒子进行了进一步表征。此外，还评估了银纳米粒子对大肠杆菌和金黄色葡萄球菌的抗菌活性。总之，该研究成功地合成了银纳米粒子，并深入了解了影响其质量特性的相关因素，同时显著提高了抗菌活性，带来了有益的效果。
