Tipping P G, Holdsworth S R
Am J Pathol. 1986 Jul;124(1):10-7.
The temporal relationships of macrophage accumulation, glomerular fibrin deposition, the expression of glomerular procoagulant activity (PCA), and Factor VIII antigen deposition were studied in rabbits in which antiglomerular basement membrane antibody-induced glomerulonephritis (anti-GBM GN) developed. The initiation of injury coincided with the accumulation of glomerular macrophages. Glomerular fibrin, assessed by immunofluorescence and by deposition of 125I-fibrinogen, paralleled the development of glomerular crescents and renal impairment. Macrophage ingress clearly preceded the deposition of 125I-fibrinogen within glomeruli. Augmented levels of PCA were present in glomeruli prior to the initiation of fibrin deposition, and peak levels coincided with the peak glomerular macrophage presence. Factor VIII related antigen was apparent late in the disease and was present mainly at the margins of fibrinous crescents. These data demonstrate that accumulation of glomerular macrophages precedes glomerular fibrin deposition in anti-GBM GN. The augmentation of PCA, coincident with the appearance of glomerular macrophages, suggests a role for macrophage PCA in the initiation of fibrin deposition within the glomerular tuft in this model.
在由抗肾小球基底膜抗体诱导发生肾小球肾炎（抗GBM GN）的家兔中，研究了巨噬细胞积聚、肾小球纤维蛋白沉积、肾小球促凝活性（PCA）表达以及VIII因子抗原沉积之间的时间关系。损伤的起始与肾小球巨噬细胞的积聚同时发生。通过免疫荧光和125I-纤维蛋白原沉积评估的肾小球纤维蛋白，与肾小球新月体的形成和肾功能损害的发展平行。巨噬细胞进入肾小球明显先于125I-纤维蛋白原在肾小球内的沉积。在纤维蛋白沉积开始之前，肾小球中PCA水平升高，峰值水平与肾小球巨噬细胞的峰值出现时间一致。VIII因子相关抗原在疾病后期明显，主要存在于纤维蛋白新月体的边缘。这些数据表明，在抗GBM GN中，肾小球巨噬细胞的积聚先于肾小球纤维蛋白沉积。PCA的增加与肾小球巨噬细胞的出现同时发生，提示在该模型中巨噬细胞PCA在肾小球毛细血管袢内纤维蛋白沉积的起始过程中起作用。
