Li Ping, Xu Junwei, Liu Lizhen, Zhang Yingnan, Liu Meili, Fan Yubo
J Biomed Nanotechnol. 2019 May 1;15(5):921-929. doi: 10.1166/jbn.2019.2749.
Electrical or mechanical stimulation has been applied widely in bone fracture treatment or bone tissue construction. The effect of combined mechanical and electrical stimulation to osteoblasts or bone tissue has not been deeply studied, which would provide a more actual simulation of microenvironment. In this study, a cell culture device that can simultaneously load mechanical and electrical stimulation on cells was self-designed and fabricated. The combined laminar shear stress and electrical stimulation could be supplied to MC3T3-E1 cells seeded on the nano-crystalline fluorine-doped tin oxide glass plate. Cell counting kit-8 assay was used to study the proliferation of MC3T3-E1 cells. The changes in alkaline phosphatase activities, genes expression of collagen-I (Col-I) and fibronectin and concentration of Col-I were also measured. Compared with single stimulation, the combined stimulation obviously enhanced proliferation, differentiation and synthesis of extracellular matrix of pre-osteoblasts in a greater extent. The combined mechanical and electrical stimulation provide a new way to promote cultivation of cells and tissues, which will be very significant in the development of tissue engineering.
电刺激或机械刺激已广泛应用于骨折治疗或骨组织构建。机械刺激与电刺激联合作用于成骨细胞或骨组织的效果尚未得到深入研究，而这种联合刺激能更真实地模拟微环境。在本研究中，自行设计并制作了一种能够同时对细胞加载机械刺激和电刺激的细胞培养装置。层流切应力和电刺激的联合作用可施加于接种在纳米晶氟掺杂氧化锡玻璃板上的MC3T3-E1细胞。采用细胞计数试剂盒-8法研究MC3T3-E1细胞的增殖情况。还检测了碱性磷酸酶活性、I型胶原（Col-I）和纤连蛋白基因表达的变化以及Col-I的浓度。与单一刺激相比，联合刺激能更显著地增强前成骨细胞的增殖、分化及细胞外基质合成。机械刺激与电刺激联合作用为促进细胞和组织培养提供了一种新途径，这在组织工程发展中将具有重要意义。
