Department of General Surgery, Tianjin Fifth Central Hospital, Tianjin, 300450, China.
Department of General Surgery, Tianjin Fifth Central Hospital, Tianjin, 300450, China.
Pathol Res Pract. 2019 Jun;215(6):152382. doi: 10.1016/j.prp.2019.03.011. Epub 2019 Mar 5.
MicroRNAs (miRNAs) play a vital role in the progress of cancer. Whereas the expression and function of miR-374a in pancreatic cancer remain largely unknown. In this study, pancreatic cancer samples and its adjacent normal tissues were obtained from 30 clinical patients with pancreatic cancer. Quantitative real-time PCR (qRT-PCR) was used to measure miR-374a and SRC Kinase Signaling Inhibitor 1 (SRCIN1) expression. Western blotting assay was performed to measure the levels of SRCIN1, E-cadherin, N-cadherin, Vimentin, Zonula occludens-1 (ZO-1) and β-catenin in PANC-1 cells. Luciferase reporter assay was conducted to confirm the direct targeting of SRCIN1 by miR-374a. Cell proliferation and migration assays were utilized to analyze the role of miR-374a in PANC-1 cells. We found that miR-374a expression was upregulated in pancreatic cancer tissues and cell lines. Over-expression of miR-374a promoted cell proliferation, migration and epithelial-mesenchymal transition (EMT) in pancreatic cancer. While, SRCIN1 expression was downregulated in pancreatic cancer tissues and cells. SRCIN1 was found to be a potential targets of miR-374a by dual-luciferase reporter assay. And SRCIN1 was down-regulated after miR-374a transfection. More than that, over-expression of SRCIN1 inhibited cell proliferation, migration and EMT in pancreatic cancer cell. Therefore, this study revealed that miR-374a promoted cell proliferation, migration and EMT via targeting SRCIN1 in pancreatic cancer.
微小 RNA（miRNAs）在癌症的进展中起着至关重要的作用。然而，miR-374a 在胰腺癌中的表达和功能仍知之甚少。在这项研究中，从 30 名胰腺癌患者中获得了胰腺癌样本及其相邻正常组织。采用定量实时 PCR（qRT-PCR）测量 miR-374a 和 SRC 激酶信号抑制剂 1（SRCIN1）的表达。通过 Western blot 测定 PANC-1 细胞中 SRCIN1、E-钙粘蛋白、N-钙粘蛋白、波形蛋白、紧密连接蛋白-1（ZO-1）和β-连环蛋白的水平。进行荧光素酶报告基因测定以确认 SRCIN1 是 miR-374a 的直接靶标。细胞增殖和迁移实验用于分析 miR-374a 在 PANC-1 细胞中的作用。我们发现 miR-374a 在胰腺癌组织和细胞系中表达上调。miR-374a 的过表达促进了胰腺癌中细胞的增殖、迁移和上皮-间充质转化（EMT）。然而，SRCIN1 在胰腺癌组织和细胞中表达下调。双荧光素酶报告基因测定发现 SRCIN1 是 miR-374a 的潜在靶标。并且转染 miR-374a 后 SRCIN1 下调。不仅如此，SRCIN1 的过表达抑制了胰腺癌细胞的增殖、迁移和 EMT。因此，本研究表明 miR-374a 通过靶向 SRCIN1 促进胰腺癌中细胞的增殖、迁移和 EMT。
