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两种均相 LDL-胆固醇检测方法在使用新鲜高甘油三酯血症血清和定量超速离心部分时的比较。

Comparison of Two Homogeneous LDL-Cholesterol Assays Using Fresh Hypertriglyceridemic Serum and Quantitative Ultracentrifugation Fractions.

机构信息

Department of Clinical Laboratory, Fukuoka University Hospital.

Department of Laboratory Medicine, Fukuoka University School of Medicine.

出版信息

J Atheroscler Thromb. 2019 Nov 1;26(11):979-988. doi: 10.5551/jat.47191. Epub 2019 Mar 19.

DOI:10.5551/jat.47191
PMID:30890680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6845691/
Abstract

AIM

The purpose of this study was to compare two homogeneous assays of low-density lipoprotein-cholesterol (LDL-C) with a modified beta quantification reference measurement for LDL-C (BQ-LDL), fractions of chylomicron (CM), very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) by quantitative ultracentrifugation in patients with hypertriglyceridemia.

METHODS

Two homogeneous LDL-C assays (LDL-C(K), Kyowa Medex and LDL-C(S), Sekisui Medical) were used to measure 198 samples of fresh anonymized leftover sera with hypertriglyceridemia (≥ 150 mg/dL). Of these, 32 samples with discrepant LDL-C levels or hypertriglyceridemia (≥ 400 mg/dL) were used for further analysis. Quantitative ultracentrifugation was used to separate samples.

RESULTS

The two homogeneous LDL-C assays had a strong correlation with each other for the samples from 198 patients with hypertriglyceridemia. LDL-C(K) and LDL-C(S) in 32 selected samples were strongly correlated with BQ-LDL. In both homogeneous assays, cholesterol in the CM and VLDL fractions was measured as part of the LDL-C. A weak correlation was found between cholesterol in the VLDL fraction and LDL-C using the two homogeneous assays, but no correlation was found with cholesterol in the CM fraction. Cholesterol in the IDL fraction was also measured as part of the LDL-C in both assays.

CONCLUSION

Both homogeneous assays partially detected cholesterol in the chylomicron and VLDL fractions, but LDL-C measured by both homogeneous assays correlated with BQ-LDL.

摘要

目的

本研究旨在比较两种均相法低密度脂蛋白胆固醇（LDL-C）检测法与改良β定量参考测量法（BQ-LDL）在测定伴有高甘油三酯血症患者的乳糜微粒（CM）、极低密度脂蛋白（VLDL）和中间密度脂蛋白（IDL）时的结果。

方法

采用两种均相 LDL-C 检测法（LDL-C(K)，协和发酵麒麟；LDL-C(S)，积水医疗）检测 198 份匿名剩余高甘油三酯血症（≥150mg/dL）患者的新鲜血清样本。其中，对 LDL-C 水平或高甘油三酯血症（≥400mg/dL）存在差异的 32 份样本进行进一步分析。采用定量超速离心法对样本进行分离。

结果

两种均相 LDL-C 检测法在检测 198 例高甘油三酯血症患者的样本时相关性较强。在 32 份选定样本中，LDL-C(K)和 LDL-C(S)与 BQ-LDL 相关性较强。两种均相检测法均将 CM 和 VLDL 中的胆固醇作为 LDL-C 的一部分进行测量。两种均相检测法中，VLDL 中的胆固醇与 LDL-C 呈弱相关，但与 CM 中的胆固醇无相关性。两种均相检测法均将 IDL 中的胆固醇作为 LDL-C 的一部分进行测量。

结论

两种均相检测法均部分检测到 CM 和 VLDL 中的胆固醇，但两种均相检测法所测 LDL-C 与 BQ-LDL 相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/285edf09dae9/jat-26-979-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/8c0ec41ab5ae/jat-26-979-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/bfbdb3f8a883/jat-26-979-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/85c4df51db74/jat-26-979-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/855f6d6796dc/jat-26-979-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/285edf09dae9/jat-26-979-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/8c0ec41ab5ae/jat-26-979-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/bfbdb3f8a883/jat-26-979-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/85c4df51db74/jat-26-979-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/855f6d6796dc/jat-26-979-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02c1/6845691/285edf09dae9/jat-26-979-g005.jpg

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Update on the laboratory investigation of dyslipidemias.血脂异常的实验室检查进展。
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