通过高效液相色谱法鉴定ε-N-单甲基赖氨酸、ε-N-二甲基赖氨酸和ε-N-三甲基赖氨酸。
Identification of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine by high-performance liquid chromatography.
作者信息
Garcia R, Hiatt W R, Jasinski S, Sypherd P S
出版信息
Appl Environ Microbiol. 1986 Jun;51(6):1355-7. doi: 10.1128/aem.51.6.1355-1357.1986.
Abstract
Dansyl derivatives of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine were resolved from other amino acids in proteins by the use of high-performance liquid chromatography. The system was tested with amino acid standard combinations as well as with acid-hydrolyzed proteins known to contain methylated residues. In all cases the methylated lysines were well resolved.
摘要
通过高效液相色谱法，从蛋白质中的其他氨基酸中分离出ε-N-单甲基、ε-N-二甲基和ε-N-三甲基赖氨酸的丹磺酰衍生物。该系统用氨基酸标准组合以及已知含有甲基化残基的酸水解蛋白进行了测试。在所有情况下，甲基化赖氨酸都能得到很好的分离。
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