Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Sao Paulo, Brazil.
Fleury Group, Sao Paulo, Brazil.
Andrology. 2019 May;7(3):341-349. doi: 10.1111/andr.12606. Epub 2019 Mar 19.
Previous studies have demonstrated an association between obesity and the decreased male fertility.
to observe the mechanisms by which obesity affects semen quality.
A prospective study was performed including 47 male volunteers, of which 27 were obese group (body mass index >30 kg/m ) and 20 were eutrophic (body mass index between 18.5 and 25 kg/m ) controls. Sperm functional analysis was performed. The remaining seminal plasma was pooled-four pools per group- and submitted to proteomic analysis by liquid chromatography coupled to tandem mass spectrometry. Groups were compared by an unpaired Student's t-test. Differentially expressed proteins were submitted to functional enrichment analysis using the online platform PantherDB.
Obese men presented decreased non-progressive motility, morphology, acrosome integrity, mitochondrial activity, and increased sperm DNA fragmentation. In proteomics analysis, 69 proteins were differentially expressed between the two groups. Among them, one protein was absent, 19 were down-regulated, 49 were up-regulated, and one was exclusive in the study group. The main functions enriched were as follows: negative regulation of the intrinsic pathway of apoptosis, activation of immune and inflammatory, antioxidant activity, among others.
molecular pathways suggest there is a causative link, and that the effector mechanisms alter sperm metabolic status and defective testicular selection 5 mechanisms.
先前的研究表明肥胖与男性生育力下降之间存在关联。
观察肥胖影响精液质量的机制。
进行了一项前瞻性研究，纳入了 47 名男性志愿者，其中 27 名肥胖组（体重指数>30kg/m²），20 名营养良好组（体重指数在 18.5 到 25kg/m²之间）作为对照。进行精子功能分析。将剩余的精浆混合，每个组四个样本池，通过液相色谱-串联质谱法进行蛋白质组学分析。通过未配对学生 t 检验比较两组。使用在线平台 PantherDB 对差异表达蛋白进行功能富集分析。
肥胖男性的非进展性运动、形态、顶体完整性、线粒体活性降低，精子 DNA 碎片化增加。在蛋白质组学分析中，两组之间有 69 种蛋白表达存在差异。其中，一种蛋白缺失，19 种蛋白下调，49 种蛋白上调，有一种蛋白仅在研究组中出现。富集的主要功能如下：凋亡内在途径的负调控、免疫和炎症激活、抗氧化活性等。
分子途径表明存在因果关系，效应机制改变了精子代谢状态和有缺陷的睾丸选择 5 种机制。
