结膜黑色素瘤中环状 RNA 的表达谱改变。
Altered expression profile of circular RNAs in conjunctival melanoma.
机构信息
Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 12, Lane 833, Zhizaoju Road, Huangpu District, Shanghai 200001, PR China.
Shanghai Key Laboratory of Orbital Diseases & Ocular Oncology, No. 12, Lane 833, Zhizaoju Road, Huangpu District, Shanghai 200001, PR China.
出版信息
Epigenomics. 2019 May;11(7):787-804. doi: 10.2217/epi-2019-0029. Epub 2019 Mar 20.
We aimed to explore the roles of circular RNA (circRNA) in conjunctival melanoma. : The altered circRNAs were identified by RNA sequencing. The function of one circRNA, circMTUS1, was explored by several experiments in conjunctival melanoma. Bioinformatic analyses and an RNA immunoprecipitation assay were performed to further study the downstream mechanism of circMTUS1. : We identified 9300 different circRNAs in conjunctival melanoma tissues compared with adjacent normal tissues. CircMTUS1 was upregulated in conjunctival melanoma. Silencing of circMTUS1 inhibited conjunctival melanoma proliferation and . CircMTUS1 may serve as an oncogene by binding to hsa-miR-622 and hsa-miR-1208 to regulate several tumor-related pathways. We demonstrated that circMTUS1 may serve as an oncogene to promote tumor proliferation.
我们旨在探索环状 RNA（circRNA）在结膜黑色素瘤中的作用。 通过 RNA 测序鉴定了改变的 circRNA。 通过几种实验探索了一个 circRNA，circMTUS1，在结膜黑色素瘤中的功能。 进行生物信息学分析和 RNA 免疫沉淀测定以进一步研究 circMTUS1 的下游机制。 与相邻的正常组织相比，我们在结膜黑色素瘤组织中鉴定出 9300 种不同的 circRNA。circMTUS1 在结膜黑色素瘤中上调。circMTUS1 的沉默抑制了结膜黑色素瘤的增殖和侵袭。circMTUS1 可能通过与 hsa-miR-622 和 hsa-miR-1208 结合来调节几种与肿瘤相关的途径，充当致癌基因。 我们证明 circMTUS1 可能作为一种致癌基因促进肿瘤增殖。
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