环状 RNA circRNA_0000285 通过调控 FUS 促进宫颈癌的发展。
Circular RNA circRNA_0000285 promotes cervical cancer development by regulating FUS.
机构信息
Department of Gynecology, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8771-8778. doi: 10.26355/eurrev_201910_19271.
OBJECTIVE
Recently, the vital role of circular RNAs (circRNAs) in human diseases has attracted much attention. The aim of this research was to verify the potential role of circRNA_0000285 in the development of cervical cancer (CC).
PATIENTS AND METHODS
CircRNA_0000285 expression in both CC cells and tissue samples was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Functional experiments were performed, including cell counting kit-8 (CCK-8) assay, cell cycle assay and transwell assay. Meanwhile, the underlying mechanism was explored through qRT-PCR and Western blot assay, respectively. In addition, the function of circRNA_0000285 was identified in vivo.
RESULTS
CircRNA_0000285 expression level was significantly higher in CC samples than that of corresponding normal tissues. Moreover, the growth and migration abilities of CC cells were significantly inhibited after circRNA_0000285 was knocked down in vitro. Furthermore, the expression of FUS was remarkably down-regulated after knockdown of circRNA_0000285. Subsequent results indicated that the expression level of FUS was positively correlated with the expression of circRNA_0000285 in CC tissues. In addition, the knockdown of circRNA_0000285 significantly inhibited the formation and metastasis of CC in nude mice.
CONCLUSIONS
CircRNA_0000285 could enhance the proliferation and metastasis of CC by up-regulating FUS, which might be a potential therapeutic target for CC treatment.
目的
环状 RNA（circRNAs）在人类疾病中的重要作用近来受到广泛关注。本研究旨在验证 circRNA_0000285 在宫颈癌（CC）发展中的潜在作用。
患者和方法
通过实时定量聚合酶链反应（qRT-PCR）检测 CC 细胞和组织样本中 circRNA_0000285 的表达。进行了细胞计数试剂盒-8（CCK-8）测定、细胞周期测定和 Transwell 测定等功能实验。同时，通过 qRT-PCR 和 Western blot 测定分别探讨了潜在机制。此外，还在体内鉴定了 circRNA_0000285 的功能。
结果
circRNA_0000285 在 CC 样本中的表达水平明显高于相应的正常组织。此外，体外敲低 circRNA_0000285 后，CC 细胞的生长和迁移能力明显受到抑制。进一步的结果表明，敲低 circRNA_0000285 后 FUS 的表达明显下调。随后的结果表明，FUS 的表达水平与 CC 组织中 circRNA_0000285 的表达水平呈正相关。此外，敲低 circRNA_0000285 显著抑制了裸鼠中 CC 的形成和转移。
结论
circRNA_0000285 通过上调 FUS 增强 CC 的增殖和转移，可能成为 CC 治疗的潜在治疗靶点。
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