Maia Filho Fernando de Souza, Fonseca Anelise de Oliveira da Silva, Valente Júlia de Souza Silveira, Baptista Cristiane Telles, Moreira Andrios da Silva, Botton Sônia de Avila, Pötter Luciana, Pereira Daniela Isabel Brayer
Programa de Pós-graduação em Parasitologia, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas - UFPel, Pelotas, RS, Brasil.
Laboratório de Micologia, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas - UFPel, Pelotas, RS, Brasil.
Rev Bras Parasitol Vet. 2019 Jan-Mar;28(1):91-96. doi: 10.1590/S1984-29612019007. Epub 2019 Mar 11.
Purpureocillium lilacinum is a nematophagous fungus used in biological control against some parasites, including Toxocara canis. This study researched the infectivity of embryonated T. canis eggs after exposure to the fungus P. lilacinum. T. canis eggs were exposed to P. lilacinum for 15 or 30 days and subsequently administered to Swiss mice (n=20). Control group consisted of mice who received T. canis embryonated eggs without fungal exposure. Forty-eight hours after infection, heart, lung, and liver from animals of each group were collected to assess larval recovery. The organs of mice that received embryonated eggs exposed to the fungus showed a lower average larval recovery (P<0.05) suggesting that exposure of T. canis eggs to P. lilacinum was able to reduce experimental infection. Under the evaluated conditions, the interaction time between the fungus and the parasite eggs was not a significant factor in larvae recovery. P. lilacinum may be considered a promising T. canis biological control agent. However, further studies are needed to determine a protocol for the use of this fungus as a biological control agent.
淡紫紫孢菌是一种用于生物防治某些寄生虫（包括犬弓首蛔虫）的食线虫真菌。本研究探讨了犬弓首蛔虫感染性虫卵经淡紫紫孢菌处理后的感染性。将犬弓首蛔虫虫卵暴露于淡紫紫孢菌15天或30天，随后接种于瑞士小鼠（n = 20）。对照组为接受未经真菌处理的犬弓首蛔虫感染性虫卵的小鼠。感染48小时后，收集每组动物的心脏、肺和肝脏以评估幼虫回收率。接受经真菌处理的感染性虫卵的小鼠器官显示平均幼虫回收率较低（P < 0.05），这表明犬弓首蛔虫虫卵经淡紫紫孢菌处理后能够降低实验性感染。在评估条件下，真菌与寄生虫虫卵的相互作用时间对幼虫回收率不是一个显著因素。淡紫紫孢菌可被认为是一种有前景的犬弓首蛔虫生物防治剂。然而，需要进一步研究以确定使用这种真菌作为生物防治剂的方案。
