Araujo Juliana Milani, Araújo Jackson Victor de, Braga Fabio Ribeiro, Ferreira Sebastião Rodrigo, Tavela Alexandre de Oliveira
Rev Bras Parasitol Vet. 2013 Jan-Mar;22(1):171-4. doi: 10.1590/s1984-29612013000100033.
The objective of this study was to use chlamydospores of the fungus Pochonia chlamydosporia (isolates VC1 and VC4) against Toxocara canis eggs in a 15-day in vitro assay. One thousand T. canis eggs were placed in Petri dishes containing 2% water agar medium with different concentrations of chlamydospores (1,000, 10,000 or 100,000) of each fungal isolate of P. chlamydosporia (treated groups) and 1,000 eggs in Petri dishes without fungus (control group). Egg counts were performed to determine the ovicidal activity, which was classified as three effect levels: type 1, type 2 and type 3. Significant differences (P < 0.01) in egg destruction were found in comparison with the control group. The highest percentage of egg destruction was found in plates containing 100,000 chlamydospores (68.5% for VC1 and 70.5% for VC4). Chlamydospores of P. chlamydosporia were effective in destroying T. canis eggs and may contribute in the future towards combating the eggs of this parasite.
本研究的目的是在一项为期15天的体外试验中，使用厚垣孢普可尼亚菌（分离株VC1和VC4）的厚垣孢子来处理犬弓首蛔虫卵。将1000个犬弓首蛔虫卵置于含有2%水琼脂培养基的培养皿中，培养基中添加不同浓度（1000、10000或100000）的厚垣孢普可尼亚菌各真菌分离株的厚垣孢子（处理组），并将1000个虫卵置于不含真菌的培养皿中（对照组）。通过计数虫卵来确定杀卵活性，杀卵活性分为三种效应水平：1型、2型和3型。与对照组相比，发现虫卵破坏存在显著差异（P < 0.01）。在含有100000个厚垣孢子的培养皿中，虫卵破坏率最高（VC1为68.5%，VC4为70.5%）。厚垣孢普可尼亚菌的厚垣孢子在破坏犬弓首蛔虫卵方面有效，未来可能有助于对抗这种寄生虫的虫卵。
