Kemball-Cook G, Barrowcliffe T W
Br J Haematol. 1986 Jul;63(3):425-34. doi: 10.1111/j.1365-2141.1986.tb07519.x.
Fractionation of a human antibody to factor VIII: Ag by immunoabsorption with factor VIII/phospholipid (PL) complex has produced two pools of labelled Fab' fragments which can be used in fluid-phase immunoradiometric assays (IRMAs). One pool binds only to the PL-binding sites on factor VIII:Ag (and thus measures only that factor VIII:Ag in a sample not bound to PL), while the second binds to other antigenic sites. Parallel assays of factor VIII-containing materials using these two pools provide estimates of the proportion of factor VIII: Ag bound to PL in those materials. Five batches each of nine brands of factor VIII concentrates from eight different manufacturers were tested for PL-bound factor VIII:Ag by this method: all contained substantial amounts, ranging from 28% to 54% of the total factor VIII:Ag present. In addition, varying amounts of the factor VIII:Ag present in both non-activated and activated prothrombin complex concentrates (PCCs) from four manufacturers were PL-bound.
通过用因子VIII/磷脂（PL）复合物进行免疫吸附对人抗因子VIII抗体进行分离，得到了两池标记的Fab'片段，可用于液相免疫放射分析（IRMA）。其中一池仅与因子VIII:Ag上的PL结合位点结合（因此仅检测样品中未与PL结合的因子VIII:Ag），而另一池则与其他抗原位点结合。使用这两池对含因子VIII的材料进行平行分析，可估算出这些材料中与PL结合的因子VIII:Ag的比例。用这种方法对来自八个不同制造商的九个品牌的因子VIII浓缩物的五个批次进行了检测，以确定与PL结合的因子VIII:Ag：所有批次均含有大量的与PL结合的因子VIII:Ag，占总因子VIII:Ag的28%至54%。此外，来自四个制造商的未激活和激活的凝血酶原复合物浓缩物（PCC）中存在的不同量的因子VIII:Ag也与PL结合。
