Lee C J, Robbins J B
Immunol Commun. 1978;7(5):503-18. doi: 10.3109/08820137809025481.
Studies were conducted on the characterization of Haemophilus influenzae type b polysaccharide (HITB-PS) and its mitogenic activity upon peripheral lymphocytes. This capsular polysaccharide was found to contain hexosamines and hexoses in addition to the main components of ribose and ribitol phosphate. The molecular weight of HITB-PS was determined as 585,000. The affinity constant of HITB-PS to unfractionated lymphocytes was 3.13 X 10(3) M-1 with 1.11 X 10(4) binding sites per cell. HITB-PS was found to be mitogenic for both human T and B lymphocytes. At optimum doses, a three to five fold increase in 3H-thymidine incorporation into T and B cells was observed. Higher than optimum doses resulted in suppression of this mitogenicity. The effect of concanavalin A (Con A) mitogenicity was detected in T and B cells treated with effective as well as suppressive doses of HITB-PS; the mitogenic activities of Con A and HITB-PS were found to be independent of each other.
开展了关于b型流感嗜血杆菌多糖（HITB - PS）的特性及其对外周淋巴细胞的促有丝分裂活性的研究。发现这种荚膜多糖除了核糖和磷酸核糖醇的主要成分外，还含有己糖胺和己糖。HITB - PS的分子量测定为585,000。HITB - PS与未分级淋巴细胞的亲和常数为3.13×10³ M⁻¹，每个细胞有1.11×10⁴个结合位点。发现HITB - PS对人T淋巴细胞和B淋巴细胞均有促有丝分裂作用。在最佳剂量下，观察到³H - 胸腺嘧啶核苷掺入T细胞和B细胞的量增加了三到五倍。高于最佳剂量会导致这种促有丝分裂活性受到抑制。在用有效剂量和抑制剂量的HITB - PS处理的T细胞和B细胞中检测到了刀豆球蛋白A（Con A）的促有丝分裂活性；发现Con A和HITB - PS的促有丝分裂活性相互独立。
