Miki M, Barden J A, dos Remedios C G
Biochim Biophys Acta. 1986 Jul 25;872(1-2):76-82. doi: 10.1016/0167-4838(86)90149-4.
Intramonomer fluorescence resonance energy transfer between the donor epsilon-ATP bound to the nucleotide site and the acceptor N-(4-dimethylamino-3,5-dinitrophenyl)maleimide (DDPM) or 4-dimethylaminophenyl-azophenyl-4'-maleimide bound to Cys-10 in G-actin was measured. The donor-acceptor distance was calculated to be about 40 A. The intermonomer energy transfer in F-actin occurring between epsilon-ADP and DABMI was also measured. The radial coordinate of Cys-10 was calculated to be 25 A based on the helical symmetry of F-actin and the recently calculated radial coordinate of the nucleotide binding site in F-actin i.e. 25 A (Miki, M., Hambly, B. and dos Remedios, C.G. (1986) Biochim. Biophys. Acta 871, 137-141). (The assumption has been made in calculating these distances that the energy donor and acceptor rotate rapidly relative to the fluorescence lifetime.) Corresponding distances separating the donor nucleotide in one monomer from acceptors on Cys-10 in the first and second nearest neighbours in F-actin are 39-40 A and 41-43 A.
测量了与核苷酸位点结合的供体ε-ATP和与G-肌动蛋白中Cys-10结合的受体N-(4-二甲基氨基-3,5-二硝基苯基)马来酰亚胺(DDPM)或4-二甲基氨基苯基-偶氮苯基-4'-马来酰亚胺之间的单体内部荧光共振能量转移。计算得出供体-受体距离约为40埃。还测量了F-肌动蛋白中ε-ADP和DABMI之间发生的单体间能量转移。根据F-肌动蛋白的螺旋对称性以及最近计算出的F-肌动蛋白中核苷酸结合位点的径向坐标即25埃（Miki, M., Hambly, B.和dos Remedios, C.G. (1986) Biochim. Biophys. Acta 871, 137 - 141），计算出Cys-10的径向坐标为25埃。（在计算这些距离时假设能量供体和受体相对于荧光寿命快速旋转。）在F-肌动蛋白中，一个单体中的供体核苷酸与第一和第二近邻单体中Cys-10上的受体之间的相应距离分别为39 - 40埃和41 - 43埃。
