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F-肌动蛋白丝中核苷酸结合位点之间的荧光能量转移。

Fluorescence energy transfer between nucleotide binding sites in an F-actin filament.

作者信息

Miki M, Hambly B D, dos Remedios C G

出版信息

Biochim Biophys Acta. 1986 Jun 5;871(2):137-41. doi: 10.1016/0167-4838(86)90166-4.

DOI:10.1016/0167-4838(86)90166-4
PMID:3707968
Abstract

Fluorescence energy transfer between nucleotide binding sites in an F-actin filament was measured using 1-N6-ethenoadenosine diphosphate (epsilon-ADP) as a fluorescent donor and 2'(or 3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate (TNP-ADP) as an acceptor, both of which were bound to F-actin. Taking into consideration the helical structure of the F-actin filament, the radial coordinate of the nucleotide binding site was calculated to be 25 A, which corresponds to a distance between these sites along the long-pitch helix of 56.3 A and along the genetic helix of 56.7 A.

摘要

使用1-N6-乙烯基腺苷二磷酸（ε-ADP）作为荧光供体，2'（或3'）-O-（2,4,6-三硝基苯基）腺苷5'-二磷酸（TNP-ADP）作为受体，测量F-肌动蛋白丝中核苷酸结合位点之间的荧光能量转移，这两种物质均与F-肌动蛋白结合。考虑到F-肌动蛋白丝的螺旋结构，计算出核苷酸结合位点的径向坐标为25埃，这对应于这些位点沿长螺距螺旋的距离为56.3埃，沿遗传螺旋的距离为56.7埃。

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