a Department of Gastroenterology , Renmin Hospital of Wuhan University, Hubei Key Laboratory of Digestive System Disease , Wuhan , Hubei , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):767-775. doi: 10.1080/21691401.2019.1577880.
Colon cancer is one of the most commonly diagnosed and deadly cancers worldwide. Further understanding of the biological mechanisms is important for exploring the molecular biomarkers and therapeutic targets of this disease. Dysregulation of long non-coding RNAs (lncRNAs) has been reported to be associated with the development and progression of various cancers. XIAP-AS1 is a novel lncRNA, which can regulate apoptosis in gastric cancer cells. However, the role of XIAP-AS1 in colorectal cancer (CRC) remains unclear. In this study, we found that XIAP-AS1 expression was significantly increased in CRC tissues and its expression showed a positive correlation with TNM stage and cumulative survival rate of CRC. To investigate whether XIAP-AS1 regulates the progression of CRC, we knocked down its expression in several CRC cell lines. CCK-8 assays showed that XIAP-AS1 knockdown remarkably suppressed CRC cell growth and arrested the cell cycle at the G0/G1 phase (flow cytometric analysis). Furthermore, XIAP-AS1 knockdown also remarkably blocked cell invasion of colon cancer cells by regulating the expression of EMT markers, such as E-cadherin, ZO-1, vimentin, and N-cadherin. Importantly, we found that XIAP-AS1 knockdown significantly reduced STAT3 phosphorylation. Overall, this study suggests that lncRNA XIAP-AS1 might serve as a potential oncogene for colon cancer.
结肠癌是全球最常见的诊断和致命癌症之一。进一步了解其生物学机制对于探索这种疾病的分子生物标志物和治疗靶点非常重要。长链非编码 RNA（lncRNA）的失调已被报道与各种癌症的发展和进展有关。XIAP-AS1 是一种新型的 lncRNA，可调节胃癌细胞的凋亡。然而，XIAP-AS1 在结直肠癌（CRC）中的作用尚不清楚。在这项研究中，我们发现 XIAP-AS1 在 CRC 组织中的表达明显增加，其表达与 TNM 分期和 CRC 的累积生存率呈正相关。为了研究 XIAP-AS1 是否调节 CRC 的进展，我们在几种 CRC 细胞系中敲低了其表达。CCK-8 测定表明，XIAP-AS1 敲低显著抑制 CRC 细胞生长，并使细胞周期停滞在 G0/G1 期（流式细胞术分析）。此外，XIAP-AS1 敲低还通过调节 EMT 标志物（如 E-钙粘蛋白、ZO-1、波形蛋白和 N-钙粘蛋白）的表达，显著抑制结肠癌细胞的侵袭。重要的是，我们发现 XIAP-AS1 敲低显著降低了 STAT3 的磷酸化。总之，这项研究表明 lncRNA XIAP-AS1 可能作为结肠癌的潜在癌基因。
