Department of Medical Oncology, Cancer Hospital of Fudan University, Minhang Branch, Shanghai, China.
General Surgery Department, Zhongshan-Xuhui Hospital Affiliated to Fudan University, Shanghai, China.
Cancer Med. 2020 Apr;9(7):2514-2523. doi: 10.1002/cam4.2850. Epub 2020 Feb 11.
Long noncoding RNAs play essential roles in colon cancer tumorigenesis. This study aimed to explore the potential function and molecular mechanisms of LINC00961 in colon cancer. qPCR results showed that LINC00961 was downregulated in colon cancer cells and tissues. Functional assays demonstrated that LINC00961 suppressed the migration and invasion of colon cancer cells in vitro. LINC00961 functioned as an endogenous sponge for miR-223-3p in colon cancer cells. SOX11 was confirmed as a target gene of miR-223-3p. The effect of miR-223-3p on colon cancer cells was then investigated. MiR-223-3p inhibition enhanced their migration and invasion. The effect of SOX11 on colon cancer cells was studied. SOX11 overexpression inhibited the invasion of colon cancer cells. LINC00961 acted as an anti-oncogene and upregulated SOX11 expression by functioning as a miR-223-3p sponge. This research revealed the molecular mechanism of LINC00961 in colon cancer. LINC00961 might act as a potential diagnostic biomarker and therapeutic target for further clinical treatments.
长链非编码 RNA 在结肠癌肿瘤发生中发挥重要作用。本研究旨在探讨 LINC00961 在结肠癌中的潜在功能和分子机制。qPCR 结果显示，LINC00961 在结肠癌细胞和组织中下调。功能测定表明，LINC00961 抑制结肠癌细胞的体外迁移和侵袭。LINC00961 在结肠癌细胞中作为 miR-223-3p 的内源性海绵发挥作用。SOX11 被确认为 miR-223-3p 的靶基因。然后研究了 miR-223-3p 对结肠癌细胞的影响。抑制 miR-223-3p 增强了它们的迁移和侵袭。研究了 SOX11 对结肠癌细胞的影响。SOX11 过表达抑制了结肠癌细胞的侵袭。LINC00961 作为反癌基因发挥作用，通过作为 miR-223-3p 的海绵而上调 SOX11 表达。这项研究揭示了 LINC00961 在结肠癌中的分子机制。LINC00961 可能作为一种潜在的诊断生物标志物和治疗靶点，用于进一步的临床治疗。
