低密度脂蛋白的巯基化及其与L2C白血病淋巴细胞的相互作用。
Thiolation of low-density lipoproteins and their interaction with L2C leukemic lymphocytes.
作者信息
Vidal M, Sainte-Marie J, Philippot J, Bienvenue A
出版信息
Biochimie. 1986 May;68(5):723-30. doi: 10.1016/s0300-9084(86)80166-3.
PMID:3089330
Abstract
We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL), the affinity of thiolated LDL for the specific receptors and their further internalization by L2C lymphocytes are preserved.
摘要
我们在此展示一种将巯基（SH）偶联至低密度脂蛋白（LDL）表面的新方法。该方法使用高半胱氨酸硫内酯（HCTL），它能以非常温和的方式与赖氨酸残基反应，并允许选择每个LDL结合的SH数量。在我们的实验条件下（8个SH/LDL），硫醇化LDL对特定受体的亲和力及其被L2C淋巴细胞的进一步内化得以保留。
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