低密度脂蛋白介导脂质体在体外靶向白血病淋巴细胞。
LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro.
作者信息
Vidal M, Sainte-Marie J, Philippot J R, Bienvenue A
出版信息
EMBO J. 1985 Oct;4(10):2461-7. doi: 10.1002/j.1460-2075.1985.tb03957.x.
Abstract
We describe a method for the covalent coupling of low-density lipoproteins (LDL) to the surface of small unilamellar vesicles, and the delivery of the liposome content to leukemic L2C lymphocytes in vitro. We demonstrate the stability of the linkage between LDL and liposomes, the preservation of vesicle integrity and the affinity of the LDL for their specific receptors after the coupling reaction. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted liposomes, and delivered into the cytoplasm of leukemic L2C lymphocytes by the LDL pathway, as demonstrated by the lethal effect on cells measured by 51chromium-release assay.
摘要
我们描述了一种将低密度脂蛋白（LDL）共价偶联到小单层囊泡表面的方法，以及在体外将脂质体内容物递送至白血病L2C淋巴细胞的方法。我们证明了LDL与脂质体之间连接的稳定性、囊泡完整性的保留以及偶联反应后LDL对其特异性受体的亲和力。潮霉素B是一种蛋白质合成的非渗透性抑制剂，被包裹在靶向脂质体中，并通过LDL途径递送至白血病L2C淋巴细胞的细胞质中，这通过51铬释放试验测量的对细胞的致死作用得以证明。
相似文献
1
LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro.低密度脂蛋白介导脂质体在体外靶向白血病淋巴细胞。
EMBO J. 1985 Oct;4(10):2461-7. doi: 10.1002/j.1460-2075.1985.tb03957.x.
2
Characterization of low density lipoprotein receptors in freshly isolated leukemic guinea pig lymphocytes (L2C).新鲜分离的白血病豚鼠淋巴细胞（L2C）中低密度脂蛋白受体的特性分析
J Recept Res. 1985;5(2-3):171-92. doi: 10.3109/10799898509041878.
3
Thiolation of low-density lipoproteins and their interaction with L2C leukemic lymphocytes.低密度脂蛋白的巯基化及其与L2C白血病淋巴细胞的相互作用。
Biochimie. 1986 May;68(5):723-30. doi: 10.1016/s0300-9084(86)80166-3.
4
Comparison of the internalization efficiency of LDL and transferrin receptors on L2C guinea pig lymphocytes.L2C豚鼠淋巴细胞上低密度脂蛋白（LDL）和转铁蛋白受体内化效率的比较。
FEBS Lett. 1986 Feb 17;196(2):242-6. doi: 10.1016/0014-5793(86)80255-1.
5
Internalization of low-density-lipoprotein-specific receptors in leukemic guinea pig lymphocytes.
Eur J Biochem. 1986 Aug 1;158(3):569-74. doi: 10.1111/j.1432-1033.1986.tb09792.x.
6
Plasma lipoproteins of leukemic guinea pigs (L2C) can regulate cholesterol biosynthesis by lymphocytes of normal guinea pigs. A comparative study of plasma lipoproteins of normal and neoplastic animals.
Eur J Biochem. 1981 Jun;117(1):219-24. doi: 10.1111/j.1432-1033.1981.tb06325.x.
7
Effects of benzyl alcohol on transferrin and low density lipoprotein receptor mediated endocytosis in leukemic guinea pig B lymphocytes.
FEBS Lett. 1990 Mar 12;262(1):13-6. doi: 10.1016/0014-5793(90)80141-5.
8
Regulation of cholesterol biosynthesis by normal and leukemic (L2C) guinea pig lymphocytes.正常和白血病（L2C）豚鼠淋巴细胞对胆固醇生物合成的调节
Proc Natl Acad Sci U S A. 1977 Mar;74(3):956-60. doi: 10.1073/pnas.74.3.956.
9
Interactions of human lymphoblasts with targeted vesicles containing Sendai virus envelope proteins.人类淋巴母细胞与含有仙台病毒包膜蛋白的靶向囊泡之间的相互作用。
Exp Cell Res. 1989 Nov;185(1):122-31. doi: 10.1016/0014-4827(89)90042-6.
10
Regulation of cholesterol biosynthesis at a stage after the 3-hydroxy-3-methylglutaryl-CoA reductase step, in normal and leukemic (L2C) guinea pig lymphocytes.在正常和白血病（L2C）豚鼠淋巴细胞中，在3-羟基-3-甲基戊二酰辅酶A还原酶步骤之后的一个阶段对胆固醇生物合成的调节。
Biochim Biophys Acta. 1987 Sep 25;921(2):405-10. doi: 10.1016/0005-2760(87)90043-9.
引用本文的文献
1
Application of advances in endocytosis and membrane trafficking to drug delivery.内吞作用和膜转运的进展在药物递送上的应用。
Adv Drug Deliv Rev. 2020;157:118-141. doi: 10.1016/j.addr.2020.07.026. Epub 2020 Aug 3.
2
Co-encapsulating the fusogenic peptide INF7 and molecular imaging probes in liposomes increases intracellular signal and probe retention.将促融合肽INF7和分子成像探针共包封于脂质体中可增强细胞内信号并提高探针保留率。
PLoS One. 2015 Mar 27;10(3):e0120982. doi: 10.1371/journal.pone.0120982. eCollection 2015.
3
Targeting receptor-mediated endocytotic pathways with nanoparticles: rationale and advances.靶向受体介导的内吞途径的纳米颗粒：原理和进展。
Adv Drug Deliv Rev. 2013 Jan;65(1):121-38. doi: 10.1016/j.addr.2012.09.041. Epub 2012 Sep 29.
本文引用的文献
1
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.人血清中超离心分离的脂蛋白的分布及化学组成
J Clin Invest. 1955 Sep;34(9):1345-53. doi: 10.1172/JCI103182.
2
Leukemia in guinea-pigs.豚鼠白血病
Am J Pathol. 1954 Mar-Apr;30(2):337-59.
3
Action of membrane-active compounds on mammalian cells. Permeabilization of human cells by ionophores to inhibitors of translation and transcription.膜活性化合物对哺乳动物细胞的作用。离子载体使人类细胞对翻译和转录抑制剂通透化。
Eur J Biochem. 1980 Aug;109(2):535-40. doi: 10.1111/j.1432-1033.1980.tb04825.x.
4
Cell-specific drug transfer from liposomes bearing monoclonal antibodies.携带单克隆抗体的脂质体介导的细胞特异性药物传递
Nature. 1981;293(5829):226-8. doi: 10.1038/293226a0.
5
Plasma lipoproteins of leukemic guinea pigs (L2C) can regulate cholesterol biosynthesis by lymphocytes of normal guinea pigs. A comparative study of plasma lipoproteins of normal and neoplastic animals.
Eur J Biochem. 1981 Jun;117(1):219-24. doi: 10.1111/j.1432-1033.1981.tb06325.x.
6
Liposome-cell interactions. A study of the interactions of liposomes containing entrapped anti-cancer drugs with the EMT6, S49 and AE1 (transport-deficient) cell lines.脂质体与细胞的相互作用。对包封有抗癌药物的脂质体与EMT6、S49和AE1（转运缺陷型）细胞系之间相互作用的研究。
Biochim Biophys Acta. 1981 May 6;643(2):346-62. doi: 10.1016/0005-2736(81)90080-8.
7
Immunotoxins: hybrid molecules of monoclonal antibodies and a toxin subunit specifically kill tumour cells.免疫毒素：单克隆抗体与毒素亚基的杂交分子可特异性杀死肿瘤细胞。
Nature. 1981 Mar 12;290(5802):145-6. doi: 10.1038/290145a0.
8
Differential endocytosis of T and B lymphocyte surface molecules evaluated with antibody-bearing fluorescent liposomes containing methotrexate.用含甲氨蝶呤的载抗体荧光脂质体评估T和B淋巴细胞表面分子的差异性内吞作用。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4148-52. doi: 10.1073/pnas.79.13.4148.
9
Monoclonal antibody covalently coupled to liposomes: specific targeting to cells.共价偶联至脂质体的单克隆抗体：对细胞的特异性靶向作用。
J Supramol Struct Cell Biochem. 1981;16(3):243-58. doi: 10.1002/jsscb.1981.380160305.
10
Liposome-mediated DNA transfer in eukaryotic cells. Dependence of the transfer efficiency upon the type of liposomes used and the host cell cycle stage.脂质体介导的真核细胞DNA转移。转移效率对所用脂质体类型和宿主细胞周期阶段的依赖性。
Biochim Biophys Acta. 1982 Oct 11;721(2):185-90. doi: 10.1016/0167-4889(82)90067-2.
Zotero 插件