从软骨体支原体中鉴定新的 DNA 相关蛋白。

Identification of new DNA-associated proteins from Waddlia chondrophila.

机构信息

Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland.

Department Microbiology and Molecular Medicine, Institute of Genetics & Genomics in Geneva (iGE3), University of Geneva, Geneva, Switzerland.

出版信息

Sci Rep. 2019 Mar 20;9(1):4885. doi: 10.1038/s41598-019-40732-1.

DOI:10.1038/s41598-019-40732-1

PMID:30894592

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6426960/

Abstract

Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.

摘要

衣原体的转录调控仍知之甚少。造成这种差距的主要原因是直到最近才出现遗传工具。我们使用肝素层析结合质谱法发现了一种衣原体相关细菌沃氏衣原体的三个新的潜在 DNA 相关蛋白（Wcw_0377、Wcw_1456 和 Wcw_1460）。通过 ChIP-seq 分析，我们确定了这三个蛋白的调控图谱，并表明 Wcw_0377 可以结合整个基因组，而 Wcw_1456 和 _1460 具有广泛的调控基因。Wcw_1456 和 Wcw_1460 与 RpoD（σ）相互作用，成为潜在的 RpoD 调节剂。另一方面，Wcw_0377 能够进入宿主细胞核，在那里它可能通过其假定的染色质重塑 SWIB/MDM2 结构域与真核组蛋白相互作用。