抗促红细胞生成素抗体的产生与人类疟疾及疟疾相关贫血无关。
Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans.
作者信息
Addai-Mensah Otchere, Gyamfi Daniel, Amponsah Francis Agyei, Annani-Akollor Max Efui, Danquah Kwabena Owusu, Boateng Lillian, Owiredu Eddie-Williams, Afriyie Edward Y, Duneeh Richard Vikpebah, Asare Renate, Ofosu Ntiamoah David, Boateng Richard
机构信息
Department of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
St. John of God Hospital, Duayaw Nkwanta, Sunyani, Ghana.
出版信息
ScientificWorldJournal. 2019 Feb 14;2019:5398732. doi: 10.1155/2019/5398732. eCollection 2019.
INTRODUCTION
The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans.
METHODS
A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method.
RESULTS
Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032).
CONCLUSION
Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia.
引言
尽管有人提出，寄生和未寄生红细胞的破坏增加以及红细胞生成不足，但疟疾相关性贫血的病理生理学尚未完全明了。循环抗促红细胞生成素（抗EPO）抗体也与小鼠的疟疾及疟疾相关性贫血有关。然而，尚未在人类中对这种关联进行研究。因此，本研究确定了抗EPO抗体产生的患病率，并评估了其与人类疟疾及疟疾相关性贫血的关联。
方法
总共招募了86名1至10岁的儿童（57名患疟疾儿童作为病例组，29名健康儿童作为对照组），他们均为加纳杜阿亚瓦恩夸塔的居民，参与了这项病例对照研究。采集静脉血用于制作疟疾显微镜检查的厚薄血膜、通过自动血液分析仪进行全血细胞计数，并采用夹心ELISA法测定抗促红细胞生成素抗体和促红细胞生成素。
结果
在招募的86名参与者中，只有3人（3.5%）抗EPO抗体呈阳性；病例组为2.3%，对照组为1.2%。病例组和对照组在抗EPO抗体产生方面无关联。促红细胞生成素浓度在疟疾相关性贫血受试者中显著更高（p = 0.032）。
结论
抗促红细胞生成素抗体与人类疟疾感染及疟疾相关性贫血无关。促红细胞生成素浓度与疟疾相关性贫血有关。
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