Chankong Thaneeya, Chotjumlong Pareena, Sastraruji Thanapat, Pongsiriwet Surawut, Iamaroon Anak, Krisanaprakornkit Suttichai
Center of Excellence in Oral and Maxillofacial Biology, Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
J Dent Sci. 2016 Sep;11(3):238-244. doi: 10.1016/j.jds.2015.12.002. Epub 2016 Apr 8.
BACKGROUND/PURPOSE: Although some studies have shown induction of cyclooxygenase 2 (COX-2) in oral lichen planus (OLP), an association between COX-2 upregulation and OLP clinical severity has not been investigated. Therefore, we aimed to compare COX-2 expression in OLP with that in normal oral tissues, and to determine correlations between COX-2 expression and both clinical criteria and visual analog scale (VAS) scores.
COX-2 expression was studied in 25 OLP and 13 normal oral tissues by immunohistochemistry. Both clinical criteria and VAS scores were used to evaluate the clinical severity of OLP. The differences in COX-2 expression between OLP and normal tissues, and the correlations between COX-2 expression and clinical severity were determined by the nonparametric statistical tests.
COX-2 expression was significantly increased in OLP epithelium when compared with normal epithelium (P < 0.001), and intense COX-2 staining in inflammatory infiltrates was observed in the OLP lamina propria. COX-2 expression in OLP epithelium and inflammatory infiltrates was significantly correlated with the clinical criteria score ( = 0.428, P = 0.007, and = 0.681, P < 0.001, respectively), whereas a significant correlation with the VAS score was observed only in OLP inflammatory infiltrates ( = 0.605, P < 0.001).
Enhanced COX-2 expression in both OLP epithelium and inflammatory infiltrates correlates well with the clinical severity. An association between VAS score and COX-2 expression in OLP inflammatory infiltrates suggests an important role of additional COX-2 expression from inflammation in causing pain in OLP patients.
背景/目的：尽管一些研究已表明口腔扁平苔藓（OLP）中存在环氧合酶2（COX-2）的诱导现象，但尚未对COX-2上调与OLP临床严重程度之间的关联进行研究。因此，我们旨在比较OLP与正常口腔组织中COX-2的表达情况，并确定COX-2表达与临床标准及视觉模拟量表（VAS）评分之间的相关性。
采用免疫组织化学方法研究了25例OLP组织和13例正常口腔组织中COX-2的表达情况。使用临床标准和VAS评分来评估OLP的临床严重程度。通过非参数统计检验确定OLP与正常组织中COX-2表达的差异，以及COX-2表达与临床严重程度之间的相关性。
与正常上皮相比，OLP上皮中COX-2表达显著增加（P < 0.001），且在OLP固有层的炎性浸润中观察到强烈的COX-2染色。OLP上皮和炎性浸润中COX-2表达与临床标准评分显著相关（分别为r = 0.428，P = 0.007和r = 0.681，P < 0.001），而仅在OLP炎性浸润中观察到与VAS评分有显著相关性（r = 0.605，P < 0.001）。
OLP上皮和炎性浸润中COX-2表达增强与临床严重程度密切相关。OLP炎性浸润中VAS评分与COX-2表达之间的关联表明，炎症中额外的COX-2表达在导致OLP患者疼痛方面起重要作用。
