扁平苔藓及相关损害中Ki-67、p53、α-平滑肌肌动蛋白和环氧化酶-2的表达：一项初步研究。
Expression of Ki-67, p53, α-SMA and COX-2 in lichen planus and related lesions: A pilot study.
作者信息
Sanketh D S, Kumari Karuna, Rao Roopa S, Haragannavar Vanishree C, Sarode Sachin C, Sarode Gargi S, Raj A Thirumal, Patil Shankargouda
机构信息
Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied sciences, Bangalore, India.
Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune 411018, India.
出版信息
J Oral Biol Craniofac Res. 2019 Apr-Jun;9(2):230-235. doi: 10.1016/j.jobcr.2018.02.003. Epub 2018 Feb 19.
BACKGROUND
Researchers have struggled to understand the natural history of lesions presenting with both lichenoid features and epithelial dysplasia. Thus the present study was designed to differentiate between OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features based on the expressions of ki-67, p53, COX-2, and α-SMA.
MATERIALS AND METHODS
Formalin-fixed paraffin-embedded archival specimens of OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features were subjected to immunohistochemical staining with ki-67, p53, COX-2, and α-SMA.
RESULTS
Ki-67 exhibited strong positivity in 100% (6/6) of epithelial dysplasia cases, 71.4% (5/7) of lichenoid dysplasia cases, 57.1% (4/7) of OLP cases and 60% (3/5) of OLP with dysplasia cases. Strong p53 staining was evident in more cases of lichenoid dysplasia [42.8% (3/7)], while moderate staining was more frequent in OLP cases [42.8% (3/7)] and OLP with dysplasia cases [42.8% (3/7)] and mild intensity was more frequent in epithelial dysplasia cases [50% (3/6)] followed by lichenoid dysplasia cases [42.8% (3/7)], OLP cases [28.5 (2/7)] and OLP with dysplasia cases [40% (2/5)]. COX-2 strong positivity was more frequent in cases of epithelial dysplasia cases [57.1% (4/7)] and OLP [50% (3/6)]. Strong α- SMA staining was noted more frequently in lichenoid dysplasia cases [71.4 (5/7)], followed by OLP cases [42.8% (3/7)] and OLP with dysplasia cases [60% (3/5)].
CONCLUSIONS
Ki-67, p53, α-SMA and COX-2 expression do not differentiate between OLP, LP with dysplasia and epithelial dysplasia with lichenoid features.
背景
研究人员一直难以理解同时具有苔藓样特征和上皮发育异常的病变的自然病史。因此，本研究旨在根据ki-67、p53、COX-2和α-SMA的表达来区分口腔扁平苔藓（OLP）、伴发育异常的OLP、上皮发育异常以及具有苔藓样特征的上皮发育异常。
材料与方法
对OLP、伴发育异常的OLP、上皮发育异常以及具有苔藓样特征的上皮发育异常的福尔马林固定石蜡包埋存档标本进行ki-67、p53、COX-2和α-SMA的免疫组织化学染色。
结果
Ki-67在上皮发育异常病例中100%（6/6）呈强阳性，在苔藓样发育异常病例中71.4%（5/7）呈强阳性，在OLP病例中57.1%（4/7）呈强阳性，在伴发育异常的OLP病例中60%（3/5）呈强阳性。强p53染色在更多的苔藓样发育异常病例中明显[42.8%（3/7）]，而中度染色在OLP病例[42.8%（3/7）]和伴发育异常的OLP病例[42.8%（3/7）]中更常见，轻度染色在上皮发育异常病例中更常见[50%（3/6）]，其次是苔藓样发育异常病例[42.8%（3/7）]、OLP病例[28.5（2/7）]和伴发育异常的OLP病例[40%（2/5）]。COX-2强阳性在上皮发育异常病例[57.1%（4/7）]和OLP病例[50%（3/6）]中更常见。强α-SMA染色在苔藓样发育异常病例中更常见[71.4（5/7）]，其次是OLP病例[42.8%（3/7）]和伴发育异常的OLP病例[60%（3/5）]。
结论
Ki-67、p53、α-SMA和COX-2的表达不能区分OLP、伴发育异常的扁平苔藓（LP）和具有苔藓样特征的上皮发育异常。
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