Oh Soram, Jung Hyun-Sook, Kim Hyun-Jung, Jang Ji-Hyun, Kim Duck-Su, Choi Kyoung-Kyu, Kim Sun-Young
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea.
Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea.
J Dent Sci. 2018 Jun;13(2):97-102. doi: 10.1016/j.jds.2017.10.008. Epub 2018 Feb 1.
BACKGROUND/PURPOSE: Matrix metalloproteinases (MMPs) play a crucial role in the pathogenesis of dental caries, collapse of adhesive interface, and chemical erosion of teeth. The objective of this study was to investigate the inhibitory effect of zinc on collagen degradation.
Human dentin was ground and demineralized by citric acid (pH 2.0). The demineralized ground dentin was incubated in six different media: artificial saliva (AS); 5 mg/ml doxycycline in AS; 3.33, 6.82, 13.63, and 27.26 mg/ml of zinc chloride (Zn) in AS. Each group was divided into two subgroups, and active MMP-2 was incorporated into one subgroup. Specimens were incubated for 24 h, 1 week, and 2 weeks. Collagen degradation product was assessed using ELISA. The results were analyzed using repeated measured ANOVA and Duncan's analysis (α = 0.05).
The amount of collagen degradation was the lowest in Doxy group. Zn groups showed a significant inhibitory effect in collagen degradation for all concentrations (P < 0.05). In subgroups without exogenous MMP-2, zinc-mediated inhibition increased in a concentration-dependent manner with increasing zinc concentration. The amount of collagen degradation product slightly increased with increased incubation time from 24 h to 2 weeks. However, in subgroups with exogenous MMP, the inhibitory effect of zinc on collagen degradation did not depend on zinc concentration.
All Zn groups for the four concentrations tested exhibited statistically significant inhibitory effect on collagen degradation.
背景/目的：基质金属蛋白酶（MMPs）在龋齿发病机制、黏附界面破坏及牙齿化学侵蚀过程中发挥着关键作用。本研究旨在探讨锌对胶原蛋白降解的抑制作用。
将人牙本质研磨并用柠檬酸（pH 2.0）脱矿。将脱矿后的研磨牙本质在六种不同培养基中孵育：人工唾液（AS）；AS中含5 mg/ml强力霉素；AS中含3.33、6.82、13.63和27.26 mg/ml氯化锌（Zn）。每组再分为两个亚组，其中一个亚组加入活性MMP-2。标本孵育24小时、1周和2周。使用酶联免疫吸附测定法评估胶原蛋白降解产物。结果采用重复测量方差分析和邓肯分析（α = 0.05）进行分析。
强力霉素组胶原蛋白降解量最低。所有浓度的锌组在胶原蛋白降解方面均显示出显著的抑制作用（P < 0.05）。在无外源性MMP-2的亚组中，锌介导的抑制作用随锌浓度增加呈浓度依赖性增强。从24小时到2周，随着孵育时间延长，胶原蛋白降解产物量略有增加。然而在有外源性MMP的亚组中，锌对胶原蛋白降解的抑制作用不依赖于锌浓度。
在所测试的四种浓度的所有锌组中，对胶原蛋白降解均表现出统计学上显著的抑制作用。
