Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Phytochemistry, Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic.
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Šlechtitelů 29, 78371, Olomouc, Czech Republic.
Anal Bioanal Chem. 2019 May;411(14):3069-3079. doi: 10.1007/s00216-019-01760-y. Epub 2019 Mar 20.
This study compares alternative approaches for analyzing phytocannabinoids in different plant materials. Three chromatographic analytical methods (ultra-high-performance liquid chromatography with tandem mass spectrometric detection and gas chromatography with mass spectrometric and flame ionization detection) were evaluated regarding selectivity, sensitivity, analytical accuracy, and precision. The performance of the methods was compared and all three methods were demonstrated to be appropriate tools for analyzing phytocannabinoids in cannabis. Gas chromatography coupled with mass spectrometric detection showed slightly better accuracy in determining phytocannabinoid acids, which are often difficult to quantify owing to their limited stability. Aspects of sample preparation, such as material homogenization and extraction, were also considered. A single ultrasonic-assisted ethanolic extraction of dried and powdered plant samples of cannabis was shown to be exhaustive for extracting the samples prior to analysis.
本研究比较了分析不同植物材料中植物大麻素的替代方法。三种色谱分析方法（超高效液相色谱-串联质谱检测和气相色谱-质谱和火焰离子化检测）在选择性、灵敏度、分析准确性和精密度方面进行了评估。比较了这些方法的性能，所有三种方法均被证明是分析大麻中植物大麻素的合适工具。气相色谱-质谱检测在确定植物大麻素酸方面表现出稍高的准确性，由于其稳定性有限，通常难以对其进行定量。还考虑了样品制备的各个方面，如材料均化和提取。研究表明，对于分析前的干燥和粉末状大麻植物样品，单一的超声辅助乙醇提取足以充分提取样品。
