Chemistry Department, University of British Columbia Okanagan, Kelowna, BC, Canada.
Supra Research and Development, Kelowna, BC, Canada.
Phytochem Anal. 2018 Sep;29(5):507-515. doi: 10.1002/pca.2761. Epub 2018 Mar 30.
Cannabis sativa L. (cannabis) is utilised as a therapeutic and recreational drug. With the legalisation of cannabis in many countries and the anticipated regulation of potency that will accompany legalisation, analytical testing facilities will require a broadly applicable, quantitative, high throughput method to meet increased demand. Current analytical methods for the biologically active components of cannabis (phytocannabinoids) suffer from low throughput and/or an incomplete complement of relevant phytocannabinoids.
To develop a rapid, quantitative and broadly applicable liquid chromatography-tandem mass spectrometry analytical method for 11 phytocannabinoids in cannabis with acidic and neutral character.
Bulk diffusion coefficients were calculated using the Taylor-Aris open tubular method, with four reference compounds used to validate the experimental set-up. Three columns were quantitatively evaluated using van Deemter plots and fit-to-purpose performance metrics. Low (1.2 μL ) and standard (3.6 μL ) extra-column variance ultra-high pressure liquid chromatography (UPLC) configurations were contrasted. Method performance was demonstrated with methanolic cannabis flower extracts.
Bulk diffusion coefficients and van Deemter plots for 11 phytocannabinoids are reported. The developed chromatographic method includes the challenging Δ /Δ -tetrahydrocannabinol isobars and, at 6.5 min, is faster than existing methods targeting similar panels of biologically active phytocannabinoids.
The bulk diffusion coefficients and van Deemter curves informed the development of a rapid quantitative method and will facilitate potential expansion to include additional compounds, including synthetic cannabinoids. The developed method can be implemented with low or standard extra-column variance UPLC configurations.
大麻（大麻）被用作治疗和娱乐药物。随着许多国家大麻合法化以及随之而来的预期合法化效力监管，分析测试设施将需要一种广泛适用、定量、高通量的方法来满足需求的增加。目前用于大麻生物活性成分（植物大麻素）的分析方法存在通量低和/或缺乏相关植物大麻素的问题。
开发一种快速、定量且广泛适用于具有酸性和中性特征的大麻中 11 种植物大麻素的液相色谱-串联质谱分析方法。
使用 Taylor-Aris 开管法计算体扩散系数，使用四个参考化合物验证实验装置。使用 van Deemter 图和适合目的的性能指标对三根柱子进行定量评估。对比了低（1.2 μL）和标准（3.6 μL）柱外体积超高效液相色谱（UPLC）配置。用甲醇大麻花提取物验证了方法性能。
报告了 11 种植物大麻素的体扩散系数和 van Deemter 图。所开发的色谱方法包括具有挑战性的 Δ /Δ -四氢大麻酚异构体，并且在 6.5 分钟内比针对类似生物活性植物大麻素组的现有方法更快。
体扩散系数和 van Deemter 曲线为快速定量方法的开发提供了信息，并将有助于包括合成大麻素在内的其他化合物的潜在扩展。该开发方法可与低或标准柱外体积 UPLC 配置一起实施。
