Romero Romero J Lucina, Carver Gavriela Dena, Arce Johnson Patricio, Perry Keith L, Thompson Jeremy R
Departamento de Biotecnología Agrícola, Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Blvd. Juan de Dios Bátiz Paredes No. 250, San Joachín, C.P. 81101, Guasave, Sinaloa, Mexico.
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
Arch Virol. 2019 May;164(5):1453-1457. doi: 10.1007/s00705-019-04207-y. Epub 2019 Mar 20.
Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.
葡萄藤红斑点病毒（GRBV）是一种在北美具有重大葡萄栽培意义的新出现病毒。在此，我们报告了一种用于GRBV即时检测的简单方案的开发。无需提取核酸；相反，只需用无菌移液器吸头刺破完整的植株，然后将其在无菌蒸馏水中孵育，以在环介导等温扩增（LAMP）反应中提供样品模板。该方法的灵敏度比传统PCR高10000倍，每个样品成本不到一美元，从采样到读出只需半个多小时即可完成。
