Institute of Endocrinology and Felsenstein Medical Research Center, Petach Tikva, Israel.
Rabin Medical Center, Petach Tikva, 49100, Israel.
Pituitary. 2019 Aug;22(4):344-352. doi: 10.1007/s11102-019-00955-x.
To test if the antibody array strategy could be utilized to simultaneously detect the secretion of multiple growth factors by human pituitary GH-adenomas and to measure octreotide-induced alterations.
Specimens of human pituitary adenomas were cultured and incubated with or without octreotide for 24 h. Conditional media were analyzed by human growth factor antibody array and VEGF concentrations were measured by ELISA. Media were also analyzed for GH concentrations. p21 expression levels were examined by Western blot of the specimens lysates.
The antibody arrays successfully identified growth factors secreted by GH-adenomas in vitro. Octreotide treatment induced both elevations and reductions in growth factors secretion. GH response to octreotide was measured, and in this small-sized study resistant and sensitive GH-adenomas presented with no unique secretome pattern of each of the groups. Octreotide-induced VEGF alterations analyzed by the antibody array and by ELISA were not fully matched.
This study suggests that the broad proteomic strategy of antibody arrays may be utilized to study the growth factors secretion pattern of GH-adenomas and its regulation by somatostatin analogs or other compounds.
检测抗体芯片策略是否可用于同时检测人垂体 GH 腺瘤分泌的多种生长因子，并测量奥曲肽诱导的变化。
将人垂体腺瘤标本进行培养和孵育，分别加入或不加入奥曲肽 24 小时。通过人生长因子抗体芯片分析条件培养基，并通过 ELISA 测量 VEGF 浓度。还分析了 GH 浓度。通过对标本裂解物进行 Western blot 检测 p21 表达水平。
抗体芯片成功鉴定了 GH 腺瘤体外分泌的生长因子。奥曲肽治疗诱导了生长因子分泌的升高和降低。测量了 GH 对奥曲肽的反应，在这项小规模研究中，耐药和敏感的 GH 腺瘤并没有表现出每组特有的分泌模式。抗体芯片和 ELISA 分析的奥曲肽诱导的 VEGF 变化并不完全匹配。
本研究表明，抗体芯片的广泛蛋白质组学策略可用于研究 GH 腺瘤的生长因子分泌模式及其受生长抑素类似物或其他化合物的调节。
