The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Road 101, Xuzhou, 221116, Shanghai, China.
Jiangsu Normal University - Edinburgh University, Centre for Transformative Biotechnology of Medicinal and Food Plants, Jiangsu Normal University, 101 Shanghai Road, Xuzhou, People's Republic of China.
Environ Sci Pollut Res Int. 2019 May;26(13):13275-13285. doi: 10.1007/s11356-019-04703-3. Epub 2019 Mar 21.
The aim of this study was to investigate how the microbial community structure adapts during the start-up phase and how the C fractionation of biogas reflects the microbial population dynamics in two parallel swine manure-fed anaerobic digesters. Two swine manure-fed reactors for the start-up of continuously stirred tank reactors at mesophilic condition were evaluated. Changes in community structure were monitored using 16S rRNA high-throughput sequencing to measure the abundance of fermenting bacteria and methanogens. Digesters with relatively stable Methanosarcinaceae started up successfully and contained high gas production and low levels of propionate. In contrast, the digester that experienced a difficult start-up period had reduced Methanosarcinaceae along with accumulated propionate and low gas production. Specific gas production, specific methane production, and C fractionation of biogas were influenced significantly by Methanosarcinaceae, Methanobacteriaceae, and Clostridiaceae, indicating that the C fractionation of biogas had significant potential to reflect microbial population changes and digester performance during the start-up period.
本研究旨在探究微生物群落结构在启动阶段如何适应，以及沼气中的 C 分馏如何反映两个平行的猪粪 fed 厌氧消化器中微生物种群动态。评估了两种用于在中温条件下启动连续搅拌罐反应器的猪粪 fed 反应器。使用 16S rRNA 高通量测序监测群落结构变化，以测量发酵细菌和产甲烷菌的丰度。相对稳定的 Methanosarcinaceae 的消化器成功启动，并产生了较高的气体产量和较低的丙酸水平。相比之下，经历了困难启动期的消化器，伴随着丙酸的积累和低气体产量，Methanosarcinaceae 减少了。沼气的特定产气量、特定甲烷产量和 C 分馏受到 Methanosarcinaceae、Methanobacteriaceae 和 Clostridiaceae 的显著影响，表明沼气的 C 分馏在启动期间具有显著反映微生物种群变化和消化器性能的潜力。
