Wang Chaoying, Zhang Chengsheng, Xu Junnv, Li Yongfu, Wang Jie, Liu Hui, Liu Yueli, Chen Zhong, Lin Haifeng
Department of Medical Oncology, The Second Affiliated Hospital of Hainan Medical University, Haikou, China.
Department of Nursing, The Second Affiliated Hospital of Hainan Medical University, Haikou, China.
Mol Genet Genomic Med. 2019 May;7(5):e644. doi: 10.1002/mgg3.644. Epub 2019 Mar 20.
Interleukin-1 receptor 2 (IL-1R2), as an anti-inflammatory cytokine, is involved in the pathogenesis and progression of lung cancer. However, the role of IL-1R2 polymorphisms in patients with lung cancer has yet to be fully elucidated.
Six single-nucleotide polymorphisms (SNPs) in IL-1R2 were genotyped in 259 patients and 346 healthy controls. We used the chi-squared test, genetic model analysis, Haploview analysis, and multifactor dimensionality reduction (MDR) to evaluate the potential association between IL-1R2 polymorphisms and lung cancer susceptibility. Bioinformatics analyses were conducted to analyze the expression level of IL-1R2 and its association with the overall survival of lung cancer.
Our results found that rs3218977-GG was associated with a decreased risk of lung cancer (odds ratio [OR] = 0.39; 95% confidence interval [CI]: 0.17-0.87; p = 0.023), and rs2072472 had a significant risk-increasing effect in the dominant model (AG + GG vs. AA: OR = 1.54; 95% CI: 1.09-2.20; p = 0.015). The MDR model also revealed that rs2072472 is the most influential risk factor of lung cancer (testing accuracy = 0.543; cross-validation consistency = 10/10; p = 0.032). In addition, our results indicated that the IL-1R2 mRNA level was downregulated in lung cancer patients, whereas the high expression of IL-1R2 was related to a poor prognosis in lung cancer.
Our results suggest that genetic variants of IL-1R2 may play a role in lung cancer susceptibility. Further population and functional validations of our findings are warranted.
白细胞介素-1受体2（IL-1R2）作为一种抗炎细胞因子，参与肺癌的发病机制和进展。然而，IL-1R2基因多态性在肺癌患者中的作用尚未完全阐明。
对259例患者和346例健康对照者进行IL-1R2基因6个单核苷酸多态性（SNP）的基因分型。我们采用卡方检验、遗传模型分析、Haploview分析和多因素降维法（MDR）来评估IL-1R2基因多态性与肺癌易感性之间的潜在关联。进行生物信息学分析以分析IL-1R2的表达水平及其与肺癌总生存期的关联。
我们的研究结果发现，rs3218977-GG与肺癌风险降低相关（比值比[OR]=0.39；95%置信区间[CI]：0.17-0.87；p=0.023），而rs2072472在显性模型中具有显著的风险增加作用（AG+GG与AA相比：OR=1.54；95%CI：1.09-2.20；p=0.015）。MDR模型还显示，rs2072472是肺癌最具影响力的风险因素（检验准确性=0.543；交叉验证一致性=10/10；p=0.032）。此外，我们的研究结果表明，肺癌患者中IL-1R2 mRNA水平下调，而IL-1R2的高表达与肺癌预后不良相关。
我们的研究结果表明，IL-1R2的基因变异可能在肺癌易感性中起作用。有必要对我们的研究结果进行进一步的人群和功能验证。
