Kornienko I V, Chebotarev D A, Makhotkin M A, Grigoriev V A, Ponomareva E N, Matishov G G
Southern Scientific Center of the Russian Academy of Sciences, Rostov-on-Don, 344006 Russia.
Southern Federal University, Rostov-on-Don, 344090 Russia.
Mol Biol (Mosk). 2019 Jan-Feb;53(1):53-63. doi: 10.1134/S0026898419010063.
The control region of mitochondrial DNA (mtDNA) in sturgeon contains one to seven tandem nucleotide repeats 78-83 bp in size. Some sturgeon species are homoplasmic by the D-loop size (Acipenser nudiventris, A. oxyrinchus, A. sturio), some are mildly heteroplasmic (A. fulvescens, Huso huso) and some are markedly heteroplasmic (A. brevirostrum, A. medirostris, A. mikadoi, A. naccarii, and A. transmontanus). This work presents a comparison of the D-loop sequences associated with the termination of mtDNA replication in fish and the conservative sequences determining the termination of replication (TAS) in these organisms. It is proposed that the D-loop heteroplasmy in sturgeon may be associated with variation in the number of tandem repeat sequences, which can form stable spatial structures during mtDNA replication. In most sturgeon species with pronounced heteroplasmy, the energy levels required for the folding of tandem repeats containing variable number of repeated units differ minimally.
鲟鱼线粒体DNA（mtDNA）的控制区含有1至7个串联核苷酸重复序列，大小为78 - 83 bp。一些鲟鱼物种的D环大小是同质性的（裸腹鲟、尖吻鲟、大西洋鲟），一些是轻度异质性的（黄鲟、欧洲鳇），还有一些是明显异质性的（短吻鲟、中吻鲟、日本鲟、纳氏鲟和高首鲟）。这项工作比较了鱼类中与mtDNA复制终止相关的D环序列以及决定这些生物体复制终止的保守序列（TAS）。有人提出，鲟鱼中的D环异质性可能与串联重复序列数量的变化有关，这些序列在mtDNA复制过程中可形成稳定的空间结构。在大多数具有明显异质性的鲟鱼物种中，含有不同数量重复单元的串联重复序列折叠所需的能量水平差异最小。
