Lisitsa A V, Petushkova N A, Levitsky L I, Zgoda V G, Larina O V, Kisrieva Yu S, Frankevich V E, Gamidov S I
Orechovich Institute of Biomedical Chemistry, Moscow, 119121 Russia.
East China University of Technology, Nanchang, 330013, China.
Mol Biol (Mosk). 2019 Jan-Feb;53(1):166-176. doi: 10.1134/S0026898419010099.
Proteome profiling of human testicular biopsies was performed using tandem mass spectrometry with electrospray ionization. Protein identification results were compared for the Mascot commercial search engine, the SearchGUI noncommercial package, and their analog IdentiProt based on the open-source IdentiPy algorithm (http://hg.theorchromo.ru/identipy). A feature of IdentiPy is an automatic optimization of MS/MS search parameters. A set of protein identifications obtained with IdentiPy was consequently greater by one third than the sets with the other search engines. For the first time, an IdentiPy/IdentiProt search was conducted within the Progenesis LC-MS framework, which allows spectrum alignment, and the proteome profile obtained with alignment was compared with that obtained using the ProteoWizard converter. A total of 16 human chromosome 18 proteins were identified, including the myelin basic protein, which is not characteristic of testicular tissue.
采用电喷雾电离串联质谱法对人类睾丸活检组织进行蛋白质组分析。将蛋白质鉴定结果在 Mascot 商业搜索引擎、SearchGUI 非商业软件包及其基于开源 IdentiPy 算法（http://hg.theorchromo.ru/identipy）的类似软件 IdentiProt 之间进行比较。IdentiPy 的一个特点是对 MS/MS 搜索参数进行自动优化。因此，使用 IdentiPy 获得的一组蛋白质鉴定结果比使用其他搜索引擎获得的结果多三分之一。首次在 Progenesis LC-MS 框架内进行 IdentiPy/IdentiProt 搜索，该框架允许进行谱图比对，并将比对后获得的蛋白质组图谱与使用 ProteoWizard 转换器获得的图谱进行比较。总共鉴定出 16 种人类 18 号染色体蛋白，其中包括睾丸组织中不具有特征性的髓鞘碱性蛋白。
