Zeng M W, Wang T, Fei C P, Zou C X, Liu Y C, Gu Z Y
Department of Forensic Medicine, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China.
Institute of Judical Expertise, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
Fa Yi Xue Za Zhi. 2018 Jun;34(6):635-639. doi: 10.12116/j.issn.1004-5619.2018.06.012. Epub 2018 Dec 25.
To investigate the expression changes of the hydrogen sulfide synthases cystathionine γ-lyase （CSE）, cystathionine β-synthase （CBS）, and 3-mercaptopyruvate sulfurtransferase （3-MST）, after optic nerve crush （ONC） in rat the retina.
The rat model of ONC was established. Rats were divided into normal control, ONC, and sham control groups. Histopathologic changes in retina, the number of retinal ganglion cells （RGC） and retinal thickness of inner part （RTIP） were measured. The changes of CSE, CBS and 3-MST mRNA expression were detected with quantitative real-time PCR.
The retinal histostructure was normal in normal controls and with minor changes in sham controls, respectively. Compared with sham group, significant retina damages were found in the ONC group： a time-dependent reduction of RGC number and RTIP. Expressions of CSE, CBS and 3-MST mRNA in rat retina were detected in normal control. Compared with normal controls, the expressions of CSE, CBS and 3-MST mRNA did not show any significant changes in the sham controls. Compared with sham controls, CBS mRNA expressions showed a time-dependent increase at 3 d, 7 d and 14 d after crush in the ONC group; CSE mRNA expressions increased to the peak at 3 d and then slightly reduced at 14 d after crush; 3-MST mRNA expressions showed the trend of increase at 3 d and 7 d and then enhanced remarkably at 14 d after crush.
Hydrogen sulfide synthases CSE, CBS and 3-MST expressions were up-regulated in rat retina following ONC, which may cause an increase in local endogenous hydrogen sulfide production in the retina and a compensatory protective effect.
研究大鼠视神经挤压伤（ONC）后视网膜中硫化氢合成酶胱硫醚γ-裂解酶（CSE）、胱硫醚β-合成酶（CBS）和3-巯基丙酮酸硫转移酶（3-MST）的表达变化。
建立大鼠ONC模型。将大鼠分为正常对照组、ONC组和假手术对照组。测量视网膜的组织病理学变化、视网膜神经节细胞（RGC）数量和视网膜内层厚度（RTIP）。采用定量实时PCR检测CSE、CBS和3-MST mRNA表达的变化。
正常对照组视网膜组织结构正常，假手术对照组有轻微变化。与假手术组相比，ONC组视网膜损伤明显：RGC数量和RTIP呈时间依赖性减少。在正常对照组中检测到大鼠视网膜中CSE、CBS和3-MST mRNA的表达。与正常对照组相比，假手术对照组中CSE、CBS和3-MST mRNA的表达无明显变化。与假手术对照组相比，ONC组挤压伤后3 d、7 d和14 d时CBS mRNA表达呈时间依赖性增加；CSE mRNA表达在挤压伤后3 d升高至峰值，然后在14 d时略有下降；3-MST mRNA表达在挤压伤后3 d和7 d呈增加趋势，然后在14 d时显著增强。
ONC后大鼠视网膜中硫化氢合成酶CSE、CBS和3-MST的表达上调，这可能导致视网膜局部内源性硫化氢产生增加并具有代偿性保护作用。
