Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Oncol Rep. 2019 May;41(5):2855-2875. doi: 10.3892/or.2019.7066. Epub 2019 Mar 14.
Four phospholipase C β (PLCB) isoforms, PLCB1, PLCB2, PLCB3 and PLCB4, have been previously investigated regarding their roles in the metabolism of inositol lipids and cancer. The present study aimed to explore the association between PLCB1‑4 and hepatocellular carcinoma (HCC). Data from 212 patients with hepatitis B virus‑associated HCC were used to analyze the diagnostic and prognostic significance of PLCB genes in. A nomogram predicted the survival probability. Gene set enrichment analysis explored gene ontology terms and the metabolic pathways associated with PLCB genes. Validation of the prognostic values of PLCB genes was performed using the Gene Expression Profiling Interactive Analysis website. PLCB1 and PLCB2 were revealed to have diagnostic value for HCC (0.869 and 0.836 area under the curve, respectively; both P≤0.05). The combination analysis of these genes had an advantage over each alone (0.905 PLCB1 and PLCB2, and 0.877 PLCB1 and PLCB3 area under the curve; P≤0.05). PLCB1 was associated with overall survival (OS) and recurrence‑free survival (RFS; adjusted P=0.002 and P=0.001, respectively). A nomogram predicted survival probability of patients with HCC at 1, 3‑ and 5‑years. Gene set enrichment analysis indicated that PLCB1 and PLCB2 are involved in the cell cycle, cell division and the PPAR signaling pathway, among other functions. Validation using GEPIA revealed that PLCB1 and PLCB2 were associated with OS and PLCB1 and PLCB4 were associated with RFS. PLCB1 and PLCB2 exhibited diagnostic value for HCC and their combination had an advantage over each individually. PLCB1 has OS and RFS prognostic value for patients with HCC.
四种磷酯酶 Cβ（PLCβ）同工酶，PLCβ1、PLCβ2、PLCβ3 和 PLCβ4，先前已经被研究过它们在肌醇脂质代谢和癌症中的作用。本研究旨在探讨 PLCβ1-4 与肝细胞癌（HCC）之间的关系。利用来自 212 例乙型肝炎病毒相关性 HCC 患者的数据，分析 PLCB 基因在中的诊断和预后意义。列线图预测了生存概率。基因集富集分析探讨了与 PLCB 基因相关的基因本体论术语和代谢途径。使用基因表达谱交互式分析网站验证了 PLCB 基因的预后价值。PLCβ1 和 PLCβ2 被发现对 HCC 具有诊断价值（曲线下面积分别为 0.869 和 0.836；均 P≤0.05）。这些基因的联合分析比单独分析具有优势（PLCβ1 和 PLCβ2 的曲线下面积为 0.905，PLCβ1 和 PLCβ3 的曲线下面积为 0.877；均 P≤0.05）。PLCβ1 与总生存期（OS）和无复发生存期（RFS）相关（调整 P=0.002 和 P=0.001）。列线图预测了 HCC 患者 1、3 和 5 年的生存概率。基因集富集分析表明，PLCβ1 和 PLCβ2 参与了细胞周期、细胞分裂和 PPAR 信号通路等功能。使用 GEPIA 进行验证表明，PLCβ1 和 PLCβ2 与 OS 相关，PLCβ1 和 PLCβ4 与 RFS 相关。PLCβ1 和 PLCβ2 对 HCC 具有诊断价值，联合应用优于单独应用。PLCβ1 对 HCC 患者的 OS 和 RFS 具有预后价值。
