Kazár J, Stencl J, Loksa V, Tacovský L, Kovácová E
Czech Med. 1986;9(2):70-7.
Direct microscopic examination of cervical smears taken from gynaecological patients and stained by Giemsa and Gimenez revealed the presence of chlamydial inclusions in 2 and elementary bodies in 6 out of 40 samples tested. Attempts of demonstration of chlamydiae from cervical smears of further patients in chick embryo yolk sacs and cycloheximide-treated McCoy cells using the centrifugation of infectious inocula succeeded in 4 and 9 cases of 71 patients examined, respectively. One isolated strain behaved as Chlamydia trachomatis based on its sensitivity to sulfadiazine, absence of killing the intraperitoneally inoculated mice, demonstration of its inclusions by iodine staining and by detection of its elementary bodies by immunofluorescence technique using mouse serum directed to C. trachomatis but not C. psittaci serotypes. At the same time, 11 of 68 patient sera tested by microimmunofluorescence test contained IgG antibodies to genitourinary serotypes of C. trachomatis, suggesting the previous contact of patients with sexually-transmitted chlamydiae. The results obtained indicate that chlamydiae may pose a public health problem in women of sexually active age also in Czechoslovakia.
对从妇科患者采集的宫颈涂片进行直接显微镜检查，涂片经吉姆萨染色和吉氏染色后，在40份检测样本中，有2份发现衣原体包涵体，6份发现原体。在对另外71例患者的宫颈涂片进行检测时，分别尝试将感染接种物离心后接种于鸡胚卵黄囊和用放线菌酮处理过的 McCoy 细胞中以检测衣原体，结果在71例受检患者中，分别有4例和9例成功检测到衣原体。根据其对磺胺嘧啶的敏感性、不致死腹腔接种小鼠、碘染色显示包涵体以及用针对沙眼衣原体而非鹦鹉热衣原体血清型的小鼠血清通过免疫荧光技术检测到原体，一株分离菌株表现为沙眼衣原体。与此同时，在通过微量免疫荧光试验检测的68份患者血清中，有11份含有针对沙眼衣原体泌尿生殖血清型的IgG抗体，这表明患者此前接触过性传播的衣原体。所获结果表明，在捷克斯洛伐克，衣原体也可能对性活跃年龄的女性构成公共卫生问题。
