Dutilh B, Bebear C
Ann Biol Clin (Paris). 1985;43(3):275-8.
We have used in routine fluorescein labelled monoclonal antibodies to detect directly C. trachomatis in genital smears from 68 men with urethritis and 32 women with leukorrhoea or cervicitis. We have compared the results with those obtained by culture on cycloheximide treated McCoy cells stained by Giemsa. C. trachomatis was detected in men respectively from 31 per cent of urethral specimens by culture and 29 per cent by direct test and in women from 12 per cent of cervical specimens by culture and 9 per cent by direct test. There was agreement between the results in 92 per cent of the specimen tested. Discrepancies were observed in case of low number of inclusions in culture or low number of elementary bodies in direct test. The direct test seems a rapid and sensitive method and can be performed by many laboratories.
我们使用常规荧光素标记的单克隆抗体,直接检测68例尿道炎男性和32例白带异常或宫颈炎女性的生殖器涂片标本中的沙眼衣原体。我们将结果与通过在经环己酰亚胺处理的 McCoy 细胞上培养、并用吉姆萨染色获得的结果进行了比较。男性尿道标本中,通过培养检测到沙眼衣原体的比例为31%,直接检测为29%;女性宫颈标本中,通过培养检测到沙眼衣原体的比例为12%,直接检测为9%。在92%的检测标本中,结果一致。在培养中包涵体数量少或直接检测中原体数量少的情况下,观察到了差异。直接检测似乎是一种快速且灵敏的方法,许多实验室都可以进行。