Department of Biological Science and Health - Biology, University of Araraquara - UNIARA, Araraquara, SP, Brazil.
Medicinal Chemistry and Regenerative Medicine Group, QUIMMERA - UNIARA, Araraquara, SP, Brazil.
J Photochem Photobiol B. 2019 May;194:6-13. doi: 10.1016/j.jphotobiol.2019.03.006. Epub 2019 Mar 12.
Follicular cystic ovary disease is a common reproductive disorder in women and females of domestic animals, characterized by anovulation and the persistence of follicle is a common cause of reproductive failure in mammalian. Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism (HA), chronic anovulation and polycystic ovaries, and it is a common reproductive endocrine disease with clinical manifestations including hirsutism, acne, infertility and obesity that can affect 5-20% of women in their reproductive age. Photobiomodulation (PBM) has been investigated and used in clinical practice, related to biomodulatory influences on cellular functions in animals and humans, both in vivo and in vitro. In this study, we include endocrine and reproductive features in a rat model for PCOS and the effects of PBM on ovarian activities. Forty-five adult female Wistar rats PCOS-induced by a single dose of the estradiol valerate (EV) were used in the study. After the EV injection for PCO induction, rats were divided into 9 groups (n = 5/group) named C30, C45 and C60 (Control group), S30, S45 and S60 (PCO group) and L30, L45 and L60 (PCO/Laser group). The rats were irradiated with laser 3 times/week. The results shown that EV PCO-induced rats had increased body mass, reduced ovary mass, and reduced GSI. The plasma levels of P4 and T were increased, and the LH plasma level was decreased by PBM stimulation. The number of ovarian follicles and corpus luteum were increased, and the number of ovarian cysts was decreased by PBM stimulation. Thus, reproductive and endocrine characteristics were modulated by PBM.
卵泡囊性卵巢疾病是一种常见的女性和家畜雌性生殖障碍，其特征是排卵障碍和卵泡持续存在，是哺乳动物生殖失败的常见原因。多囊卵巢综合征（PCOS）的特征是高雄激素血症（HA）、慢性无排卵和多囊卵巢，是一种常见的生殖内分泌疾病，临床表现包括多毛症、痤疮、不孕和肥胖症，可影响 5-20%的育龄妇女。光生物调节（PBM）已在临床实践中进行了研究和应用，涉及对动物和人类细胞功能的生物调节影响，包括体内和体外。在这项研究中，我们将内分泌和生殖特征纳入多囊卵巢综合征大鼠模型中，并研究 PBM 对卵巢活动的影响。将 45 只成年雌性 Wistar 大鼠用单剂量戊酸雌二醇（EV）诱导多囊卵巢综合征（PCOS），用于这项研究。在 EV 注射诱导 PCO 后，将大鼠分为 9 组（每组 5 只），分别命名为 C30、C45 和 C60（对照组）、S30、S45 和 S60（PCO 组）和 L30、L45 和 L60（PCO/激光组）。大鼠每周接受激光照射 3 次。结果表明，EV 诱导的 PCO 大鼠体重增加、卵巢质量减少、GSI 降低。PBM 刺激可增加血浆 P4 和 T 水平，降低 LH 血浆水平。PBM 刺激可增加卵巢卵泡和黄体数量，减少卵巢囊肿数量。因此，PBM 可调节生殖和内分泌特征。
