一种低成本、便携式且易于操作的唾液尿素传感器，可用于即时检测。
A low-cost, portable and easy-operated salivary urea sensor for point-of-care application.
机构信息
Department of Photonics, College of Electrical and Computer Engineering, National Chiao Tung University, 1001, Ta Hsueh Rd., 300 Hsinchu, Taiwan.
Department of Biological Science and Technology, National Chiao Tung University, 1001, Ta Hsueh Rd., 300 Hsinchu, Taiwan; Department of Internal Medicine, Division of Nephrology, National Taiwan University Hospital Hsin-Chu Branch, 25, Ln. 442, Section 1, Jingguo Rd., 300 Hsinchu, Taiwan.
出版信息
Biosens Bioelectron. 2019 May 1;132:352-359. doi: 10.1016/j.bios.2019.03.007. Epub 2019 Mar 7.
Salivary urea was reported to be a useful biomarker to reflect the blood urea nitrogen in chronic kidney disease patients. However, as a new biomarker, enormous clinical trials are required to define the intended-use and to verify the specification. In this report, we demonstrated a low-cost easy-operated real-time sensing system (optical fiber-urea-sensing, OFUS, system) to detect salivary urea. We aim to make the system easily reproduced by the community to stimulate abundant clinical tests worldwide. The OFUS system is composed of a simple three-dimensional printed tank to link with two optical fibers, one connecting with a commercial light-emitting diode to deliver the input light signal, the other connecting with a commercial cadmium sulfide photo-conductive cell to detect the sensing signal. To allow on-site detection without any sample pretreatment, only 1 μl saliva is needed to be mixed with 10 μl urease solution and 90 μl pH indicator solution in the reaction tank and the detection time is only 20 s. A stable and reproducible calibration curve can be easily built with a detection range as 24-300 mg/dL. The OFUS system successfully detected saliva with added synthetic urea and samples from chronic kidney disease patients. A good agreement between the OFUS system and the commercial kit was obtained. A good correlation between salivary urea and the blood urea nitrogen was also confirmed.
唾液尿素被报道为一种有用的生物标志物，可反映慢性肾脏病患者的血尿素氮水平。然而，作为一种新的生物标志物，需要进行大量的临床试验来定义其预期用途并验证其规格。在本报告中，我们展示了一种低成本、易于操作的实时传感系统（光纤尿素传感系统，OFUS 系统）来检测唾液尿素。我们旨在使该系统易于被社区复制，以在全球范围内刺激丰富的临床测试。OFUS 系统由一个简单的三维打印水箱组成，用于连接两根光纤，一根与商用发光二极管相连，用于传输输入光信号，另一根与商用硫化镉光电导元件相连，用于检测传感信号。为了实现现场检测而无需任何样品预处理，只需将 1 μl 唾液与 10 μl 脲酶溶液和 90 μl pH 指示剂溶液在反应罐中混合，检测时间仅为 20 s。可以轻松建立一个稳定且可重复的校准曲线，检测范围为 24-300mg/dL。OFUS 系统成功检测了添加合成尿素的唾液和慢性肾脏病患者的样本。OFUS 系统与商业试剂盒之间获得了良好的一致性。还证实了唾液尿素与血尿素氮之间存在良好的相关性。
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