用于肌红蛋白和 miRNA-141 的即时护理点定量和灵敏检测的光纤放大器。
Optical fiber amplifier for quantitative and sensitive point-of-care testing of myoglobin and miRNA-141.
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
出版信息
Biosens Bioelectron. 2019 Mar 15;129:87-92. doi: 10.1016/j.bios.2018.12.056. Epub 2019 Jan 18.
A simple, sensitive, quantitative point-of-care testing (POCT) was developed integrating enzyme-linked DNA supersandwich amplification with optical fiber amplifier. The point-of-care (POC) assay can work in both "Turn-off" mode and "Turn-on" mode. Using myoglobin (Myo) and miRNA-141 as the model biomarker, as low as 0.5 nM Myo and 10 pM miRNA-141 could be detected. More importantly, the optical fiber amplifier, which was used as signal readout, exhibited low-cost, small size, rapid response, and easy operation. Although it did not need large equipment and professional technical personnel, the assay was successfully applied to detect Myo and miRNA-141 in 40% human serum. Since the entire process of the strategy could be done directly in the centrifugal tube, requiring a few easy steps, it showed great potential application for early diagnosis in the world, especially in developing countries or remote regions. This work provided a new avenue for developing the portable and sensitive biosensor.
一种简单、灵敏、定量的即时检测（POCT）方法，通过酶联 DNA 超三明治扩增与光纤放大器集成而开发。该即时检测（POCT）可在“关闭”模式和“开启”模式下工作。以肌红蛋白（Myo）和 miRNA-141 作为模型生物标志物，可检测到低至 0.5 nM 的 Myo 和 10 pM 的 miRNA-141。更重要的是，用作信号读出的光纤放大器具有低成本、小尺寸、快速响应和易于操作的特点。尽管该方法不需要大型设备和专业技术人员，但已成功应用于检测 40%人血清中的 Myo 和 miRNA-141。由于该策略的整个过程可以直接在离心管中完成，只需几个简单的步骤，因此它在世界范围内，特别是在发展中国家和偏远地区的早期诊断方面具有巨大的潜在应用价值。这项工作为开发便携式和灵敏的生物传感器提供了新途径。
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