Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; Department of Zoology and Entomology, College of Science, Helwan University, Cairo, Egypt.
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Saudi Arabia.
J Infect Public Health. 2019 Sep-Oct;12(5):660-665. doi: 10.1016/j.jiph.2019.03.005. Epub 2019 Mar 18.
Trypanosomiasis is a neglected tropical disease, transmitted by blood-sucking insects and can affect humans and animals, depending on the species of Trypanosoma parasite. Trypanosoma has acquired resistance to the majority of drugs used; hence, alternative medicines are required. Indigofera oblongifolia leaf extract (IOE) has been shown to treat blood stage malaria. Here, IOE was used to demonstrate its effect on Trypanosoma evansi-infected mice.
Analysis of IOE by gas chromatography-mass spectrometry showed the presence of many active components like flavonoids and phenolics. The mice were divided into three groups as follows: vehicle control, T. evansi-infected mice and T. evansi-infected-treated mice.
The findings demonstrate a significant effect of IOE treatment on T. evansi-infected mice. Parasitemia was decreased by 70%, weight loss was reduced, and splenomegaly was significantly decreased. Additionally, IOE improved the histological architecture of the spleen, as shown by the improved histological injury score post-treatment. Anemia was apparent during the course of infection in T. evansi-infected mice; this was reversed upon treatment with IOE to almost the normal level of hemoglobin and erythrocytes. Reduced glutathione and catalase were also ameliorated upon IOE treatment compared to T. evansi-infected mice.
Overall, this study shows the ameliorative role of IOE against T. evansi-induced spleen injury in mice.
锥虫病是一种被忽视的热带病，通过吸血昆虫传播，可影响人类和动物，具体取决于锥虫寄生虫的种类。锥虫已对大多数使用的药物产生了耐药性；因此，需要替代药物。菘蓝叶提取物（IOE）已被证明可治疗红内期疟疾。在这里，IOE 被用于证明其对感染伊氏锥虫的小鼠的影响。
气相色谱-质谱联用分析表明，IOE 中存在许多具有活性的成分，如类黄酮和酚类。将小鼠分为三组： vehicle 对照组、感染伊氏锥虫的小鼠组和感染伊氏锥虫治疗组。
研究结果表明 IOE 治疗对感染伊氏锥虫的小鼠有显著影响。寄生虫血症降低了 70%，体重减轻减少，脾肿大明显减少。此外，IOE 改善了脾的组织学结构，治疗后组织学损伤评分得到改善。在伊氏锥虫感染的小鼠中出现贫血；用 IOE 治疗后，血红蛋白和红细胞几乎恢复到正常水平，贫血得到逆转。与感染伊氏锥虫的小鼠相比，IOE 治疗还改善了还原型谷胱甘肽和过氧化氢酶。
总的来说，这项研究表明 IOE 对伊氏锥虫诱导的小鼠脾损伤具有改善作用。
