Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; Department of Zoology and Entomology, College of Science, Helwan University, Cairo, Egypt.
Microb Pathog. 2018 Jun;119:170-182. doi: 10.1016/j.micpath.2018.04.009. Epub 2018 Apr 6.
Malaria is still a major health problem worldwide. This study aimed to investigate the hepatoprotective role of Indigofera oblongifolia leaf extracts (ILE) against mice hepatic injury induced by Plasmodium chabaudi. Female C57BL/6 mice were treated with 100 mg/kg of ILE after infection with erythrocytes parasitized by P. chabaudi. On day 7 post-infection, the extract improved the histological alteration induced by the parasite. This was evidenced by the decreased histological index induced by ILE. Moreover, ILE was able to increase the hepatic antioxidant capacity and could significantly improve the decrease in erythrocyte count and hemoglobin content in mice blood plasma due to infection. ILE was also able to upregulate the expression of 24 genes related to metabolism and of 3 genes related to the immune response. Furthermore, the extract was able to downregulate the expression of 35 genes related to metabolism and of 82 genes related to immune response. Moreover, the microarray study showed that ILE regulated the change in gene expression induced by the parasite. Among these genes, we quantified the expression of cd209f, cyp7a1, Hsd3b5, Sult2a3, Lcn2, CcI8, Nos2, and saa3-mRNAs. These genes were regulated by ILE. Therefore, our results revealed the protective role of Indigofera oblongifolia against hepatic injury induced by blood stage malaria.
疟疾仍然是全球主要的健康问题。本研究旨在探讨菘蓝叶提取物（ILE）对伯氏疟原虫诱导的小鼠肝损伤的保护作用。感染伯氏疟原虫红细胞后，雌性 C57BL/6 小鼠用 100mg/kg 的 ILE 处理。感染后第 7 天，提取物改善了寄生虫引起的组织学改变。这一点可以从 ILE 引起的组织学指数降低得到证明。此外，ILE 能够增加肝脏的抗氧化能力，并能显著改善因感染导致的小鼠血浆中红细胞计数和血红蛋白含量的下降。ILE 还能够上调与代谢相关的 24 个基因和与免疫反应相关的 3 个基因的表达。此外，该提取物能够下调与代谢相关的 35 个基因和与免疫反应相关的 82 个基因的表达。此外，基因芯片研究表明，ILE 调节了寄生虫诱导的基因表达变化。在这些基因中，我们定量了 cd209f、cyp7a1、Hsd3b5、Sult2a3、Lcn2、CcI8、Nos2 和 saa3-mRNAs 的表达。这些基因受 ILE 调节。因此，我们的结果揭示了菘蓝对疟原虫引起的肝损伤的保护作用。
