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子宫组织驻留巨噬细胞的混合培养及其类固醇生成分子的表达特性

Mixed-Culture Propagation of Uterine-Tissue-Resident Macrophages and Their Expression Properties of Steroidogenic Molecules.

作者信息

Ogawa Kazushige, Tanida Takashi

机构信息

Laboratory of Veterinary Anatomy, Graduate School of Veterinary Science, Osaka Metropolitan University, 1-58 Rinku-Ourai-Kita, Izumisano, Osaka 598-8531, Japan.

出版信息

Biomedicines. 2023 Mar 22;11(3):985. doi: 10.3390/biomedicines11030985.

DOI:10.3390/biomedicines11030985
PMID:36979964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10046189/
Abstract

Tissue-resident macrophages (Mø) play tissue/organ-specific roles, and the physiological/pathological implications of uterine Mø in fertility and infertility are not yet fully understood. Herein, we report a simple propagation method for tissue-resident Mø by mixed culture with the respective tissue/organ-residing cells as the niche. We successfully propagated mouse uterine Mø by mixed culture with fibroblastic cells that exhibited properties of endometrial stromal cells. Propagated mouse uterine Mø were CD206- and arginase-1-positive; iNOS- and MHC-II-negative, indicating M2 polarization; and highly phagocytic, similar to endometrial Mø. Furthermore, uterine Mø were observed to express steroidogenic molecules including SRD5A1 and exhibited gap junction formation, likely with endometrial stromal cells. Accordingly, uterine Mø propagated by mixed culture may provide a new tool for studying immune-endocrine interactions related to fertility and infertility, particularly androgen's intracrine actions in preparing the uterine tissue environment to support implantation and pregnancy as well as in the etiology of endometriosis.

摘要

组织驻留巨噬细胞（Mø）发挥着组织/器官特异性作用，而子宫Mø在生育和不孕方面的生理/病理意义尚未完全明确。在此，我们报告一种通过与各自组织/器官驻留细胞作为生态位进行混合培养来繁殖组织驻留Mø的简单方法。我们通过与表现出子宫内膜基质细胞特性的成纤维细胞混合培养，成功繁殖了小鼠子宫Mø。繁殖的小鼠子宫Mø CD206和精氨酸酶-1呈阳性；诱导型一氧化氮合酶（iNOS）和主要组织相容性复合体II类分子（MHC-II）呈阴性，表明为M2极化；并且具有高度吞噬性，类似于子宫内膜Mø。此外，观察到子宫Mø表达包括5α-还原酶1（SRD5A1）在内的类固醇生成分子，并与子宫内膜基质细胞形成缝隙连接。因此，通过混合培养繁殖的子宫Mø可能为研究与生育和不孕相关的免疫-内分泌相互作用提供一种新工具，特别是雄激素在准备子宫组织环境以支持着床和妊娠以及子宫内膜异位症病因学中的内分泌作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/990eadb30f55/biomedicines-11-00985-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/20f635621c42/biomedicines-11-00985-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/6211253851d2/biomedicines-11-00985-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/a18a75c87625/biomedicines-11-00985-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/ba5c947e56ed/biomedicines-11-00985-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/38e55ec64011/biomedicines-11-00985-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/b5faa5bff444/biomedicines-11-00985-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/e99fd71bb8bf/biomedicines-11-00985-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/357d961bde84/biomedicines-11-00985-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/990eadb30f55/biomedicines-11-00985-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/20f635621c42/biomedicines-11-00985-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/6211253851d2/biomedicines-11-00985-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/a18a75c87625/biomedicines-11-00985-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/ba5c947e56ed/biomedicines-11-00985-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/38e55ec64011/biomedicines-11-00985-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/b5faa5bff444/biomedicines-11-00985-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/e99fd71bb8bf/biomedicines-11-00985-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/357d961bde84/biomedicines-11-00985-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d484/10046189/990eadb30f55/biomedicines-11-00985-g009.jpg

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