Geng L, Iwabuchi K, Sakai S, Ogasawara M, Fujita M, Ogasawara K, Kakinuma M, Good R A, Morikawa K, Onoé K
Immunology. 1986 Jul;58(3):453-7.
Synthetic tissue sites for the fourth and fifth components of complement (C4 and C5) have been investigated using allogeneic bone marrow chimaeras in mice. One group of chimaeric mice was prepared by transplanting bone marrow cells from C5-sufficient donor mice into irradiated C5-deficient recipients or vice versa, and another group was prepared by transplanting marrow cells from mice that produce high levels of C4 into irradiated recipients that are characterized by having low levels of C4 or vice versa. In such chimaeras, lymphoid cells and serum immunoglobulin allotypes were shown to be exclusively of donor origin. However, haemolytic activities of sera from the chimaeras were consistently identical with those of normal mice of the recipient strain. Similar results were obtained when the complement component levels of the sera were evaluated by double diffusion assays. C4 or C5 antigens were detected in sera of the chimaeras only when recipients were strains that are characterized by having high C4 level or were C5-sufficient mice, respectively. These findings indicate that circulating C4 or C5 complement components present in the blood are not synthesized primarily by cells that are descendants of bone marrow cells in these chimaeric mice.
利用小鼠同种异体骨髓嵌合体，对补体第四和第五成分（C4和C5）的合成组织部位进行了研究。一组嵌合小鼠是通过将来自C5充足供体小鼠的骨髓细胞移植到经辐照的C5缺陷受体中制备而成，反之亦然；另一组是通过将产生高水平C4的小鼠的骨髓细胞移植到以低水平C4为特征的经辐照受体中制备而成，反之亦然。在这类嵌合体中，淋巴细胞和血清免疫球蛋白同种异型被证明完全源自供体。然而，嵌合体血清的溶血活性始终与受体品系正常小鼠的溶血活性相同。当通过双向扩散试验评估血清中的补体成分水平时，也得到了类似结果。仅当受体分别是以高C4水平为特征的品系或C5充足的小鼠时，才能在嵌合体血清中检测到C4或C5抗原。这些发现表明，这些嵌合小鼠血液中存在的循环C4或C5补体成分并非主要由骨髓细胞的后代细胞合成。
